BD PharmingenTM Purified Mouse Anti-Human CD95

Description

Flow cytometric analysis of CD95 expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD45RO Cat. No. 561889/555493 and Purified Mouse Anti-Human CD95 Cat. No. 555671 followed by FITC Goat Anti-Mouse IgG/IgM Cat. No. 555988 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-color contour plot was derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. DX2-induced apoptosis in Fas transfectant cells line P825/FASDT151 detected by DNA fragmentation analysis. Cells were incubated alone lane 1 , in the presence of mouse IgG1 lane 2 or in the presence of DX2 at 10, 100 or 500 ng/ml lanes 3, 4 and 5, respectively . Flow cytometric analysis of CD95 expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD45RO Cat. No. 561889/555493 and Purified Mouse Anti-Human CD95 Cat. No. 555671 followed by FITC Goat Anti-Mouse IgG/IgM Cat. No. 555988 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-color contour plot was derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Show More DX2-induced apoptosis in Fas transfectant cells line P825/FASDT151 detected by DNA fragmentation analysis. Cells were incubated alone lane 1 , in the presence of mouse IgG1 lane 2 or in the presence of DX2 at 10, 100 or 500 ng/ml lanes 3, 4 and 5, respectively . Show More Flow cytometric analysis of CD95 expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD45RO Cat. No. 561889/555493 and Purified Mouse Anti-Human CD95 Cat. No. 555671 followed by FITC Goat Anti-Mouse IgG/IgM Cat. No. 555988 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-color contour plot was derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. DX2-induced apoptosis in Fas transfectant cells line P825/FASDT151 detected by DNA fragmentation analysis. Cells were incubated alone lane 1 , in the presence of mouse IgG1 lane 2 or in the presence of DX2 at 10, 100 or 500 ng/ml lanes 3, 4 and 5, respectively .