BD PharmingenTM Purified Mouse Anti-Human CEACAM5 (CD66e)

Description

Immunohistochemical analysis of CEACAM5 CD66e expression Left Panel - Immunohistochemical staining of Human Normal Colon. Formalin-fixed, paraffin-embedded sections of Human Normal Colon were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Image or Purified Mouse Anti-CEACAM5 CD66e antibody Cat. No. 568709; Right Image followed by Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550337 and Streptavidin HRP Cat. No.550946 . Antigen retrieval with BD Retrievagen A Cat. No. 550524 was performed prior to staining. Original magnification 20x. Right Panel - Immunohistochemical staining of Human Colon Cancer. Formalin-fixed, paraffin-embedded sections of Human Colon Cancer were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Image or Purified Mouse Anti-CEACAM5 CD66e antibody Cat. No. 568709; Right Image followed by Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550337 and Streptavidin HRP Cat. No.550946 . Antigen retrieval with BD Retrievagen A Cat. No. 550524 was performed prior to staining. Original magnification 20x. Flow cytometric analysis of CEACAM5 CD66e expression on BxPC-3 cells. Cells from the human BxPC-3 Adenocarcinoma, ATCC CRL-1687 cell line were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dotted line histogram or Purified Mouse Anti-CEACAM5 CD66e antibody Cat. No. 568709; solid line histogram at 0.5 mug/test, followed by Biotin Goat Anti-Mouse Ig Cat. No. 550337/553999 at 0.25 mug/test followed by PE Streptavidin Cat. No. 554061 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CEACAM5 CD66e expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Immunohistochemical analysis of CEACAM5 CD66e expression Left Panel - Immunohistochemical staining of Human Normal Colon. Formalin-fixed, paraffin-embedded sections of Human Normal Colon were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Image or Purified Mouse Anti-CEACAM5 CD66e antibody Cat. No. 568709; Right Image followed by Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550337 and Streptavidin HRP Cat. No.550946 . Antigen retrieval with BD Retrievagen A Cat. No. 550524 was performed prior to staining. Original magnification 20x. Right Panel - Immunohistochemical staining of Human Colon Cancer. Formalin-fixed, paraffin-embedded sections of Human Colon Cancer were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Image or Purified Mouse Anti-CEACAM5 CD66e antibody Cat. No. 568709; Right Image followed by Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550337 and Streptavidin HRP Cat. No.550946 . Antigen retrieval with BD Retrievagen A Cat. No. 550524 was performed prior to staining. Original magnification 20x. Show More Flow cytometric analysis of CEACAM5 CD66e expression on BxPC-3 cells. Cells from the human BxPC-3 Adenocarcinoma, ATCC CRL-1687 cell line were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dotted line histogram or Purified Mouse Anti-CEACAM5 CD66e antibody Cat. No. 568709; solid line histogram at 0.5 mug/test, followed by Biotin Goat Anti-Mouse Ig Cat. No. 550337/553999 at 0.25 mug/test followed by PE Streptavidin Cat. No. 554061 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CEACAM5 CD66e expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Show More Immunohistochemical analysis of CEACAM5 CD66e expression Left Panel - Immunohistochemical staining of Human Normal Colon. Formalin-fixed, paraffin-embedded sections of Human Normal Colon were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Image or Purified Mouse Anti-CEACAM5 CD66e antibody Cat. No. 568709; Right Image followed by Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550337 and Streptavidin HRP Cat. No.550946 . Antigen retrieval with BD Retrievagen A Cat. No. 550524 was performed prior to staining. Original magnification 20x. Right Panel - Immunohistochemical staining of Human Colon Cancer. Formalin-fixed, paraffin-embedded sections of Human Colon Cancer were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Image or Purified Mouse Anti-CEACAM5 CD66e antibody Cat. No. 568709; Right Image followed by Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550337 and Streptavidin HRP Cat. No.550946 . Antigen retrieval with BD Retrievagen A Cat. No. 550524 was performed prior to staining. Original magnification 20x. Flow cytometric analysis of CEACAM5 CD66e expression on BxPC-3 cells. Cells from the human BxPC-3 Adenocarcinoma, ATCC CRL-1687 cell line were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dotted line histogram or Purified Mouse Anti-CEACAM5 CD66e antibody Cat. No. 568709; solid line histogram at 0.5 mug/test, followed by Biotin Goat Anti-Mouse Ig Cat. No. 550337/553999 at 0.25 mug/test followed by PE Streptavidin Cat. No. 554061 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CEACAM5 CD66e expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software.