BD PharmingenTM Purified Mouse Anti-Human Cyclin E

Description

Left Figure: Western blot analysis of Cyclin E expression on HeLa cell lysate. Lysate from HeLa cells ATCC CCL-2 was probed with Purified Mouse Anti-Human Cyclin E antibody Cat. No. 551160/551159 at concentrations of 1.0 mug/mL lane 1 , 0.5 mug/mL lane 2 , and 0.25 mug/mL lane 3 . Cyclin E is identified as a band of 50 kDa. Right Figure: Immunofluorescent analysis of Cyclin E expression on HeLa cells. HeLa cells were seeded in a 96-well imaging plate at 10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and Purified Mouse Anti-Human Cyclin E antibody. The second step reagent was Alexa Fluor 555 goat anti-mouse IgG Invitrogen . The image was taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells and worked with both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure . Left Figure: Western blot analysis of Cyclin E expression on HeLa cell lysate. Lysate from HeLa cells ATCC CCL-2 was probed with Purified Mouse Anti-Human Cyclin E antibody Cat. No. 551160/551159 at concentrations of 1.0 mug/mL lane 1 , 0.5 mug/mL lane 2 , and 0.25 mug/mL lane 3 . Cyclin E is identified as a band of 50 kDa. Right Figure: Immunofluorescent analysis of Cyclin E expression on HeLa cells. HeLa cells were seeded in a 96-well imaging plate at 10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and Purified Mouse Anti-Human Cyclin E antibody. The second step reagent was Alexa Fluor 555 goat anti-mouse IgG Invitrogen . The image was taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells and worked with both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure . Show More Left Figure: Western blot analysis of Cyclin E expression on HeLa cell lysate. Lysate from HeLa cells ATCC CCL-2 was probed with Purified Mouse Anti-Human Cyclin E antibody Cat. No. 551160/551159 at concentrations of 1.0 mug/mL lane 1 , 0.5 mug/mL lane 2 , and 0.25 mug/mL lane 3 . Cyclin E is identified as a band of 50 kDa. Right Figure: Immunofluorescent analysis of Cyclin E expression on HeLa cells. HeLa cells were seeded in a 96-well imaging plate at 10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and Purified Mouse Anti-Human Cyclin E antibody. The second step reagent was Alexa Fluor 555 goat anti-mouse IgG Invitrogen . The image was taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells and worked with both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure .