BD PharmingenTM Purified Mouse anti-Human FoxP3

Description

Flow cytometric analysis of Purified anti-Human FoxP3 on resting PBMC. Human PBMC were fixed and permeabilized using working solutions of FoxP3 Buffers A and C Cat. No. 560098. see Recommended Assay Procedure , Intracellular staining with Purified Mouse anti-Human FoxP3 clone 259D/C7, Cat. No. 560044 was followed by secondary staining with APC Rat anti-Mouse IgG1 Clone X56, Cat. No. 550874 . Cells were washed and blocked with normal mouse serum and surface stained with PE anti-human CD25 Clone M-A251, Cat. No. 555432 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD25+ shown as FoxP3 vs CD25. Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of Purified anti-Human FoxP3 on resting PBMC. Human PBMC were fixed and permeabilized using working solutions of FoxP3 Buffers A and C Cat. No. 560098. see Recommended Assay Procedure , Intracellular staining with Purified Mouse anti-Human FoxP3 clone 259D/C7, Cat. No. 560044 was followed by secondary staining with APC Rat anti-Mouse IgG1 Clone X56, Cat. No. 550874 . Cells were washed and blocked with normal mouse serum and surface stained with PE anti-human CD25 Clone M-A251, Cat. No. 555432 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD25+ shown as FoxP3 vs CD25. Flow cytometry was performed on a BD FACSCalibur System. Show More Flow cytometric analysis of Purified anti-Human FoxP3 on resting PBMC. Human PBMC were fixed and permeabilized using working solutions of FoxP3 Buffers A and C Cat. No. 560098. see Recommended Assay Procedure , Intracellular staining with Purified Mouse anti-Human FoxP3 clone 259D/C7, Cat. No. 560044 was followed by secondary staining with APC Rat anti-Mouse IgG1 Clone X56, Cat. No. 550874 . Cells were washed and blocked with normal mouse serum and surface stained with PE anti-human CD25 Clone M-A251, Cat. No. 555432 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD25+ shown as FoxP3 vs CD25. Flow cytometry was performed on a BD FACSCalibur System.