BD PharmingenTM Purified Mouse Anti-Human GITR (CD357)

Description

Flow cytometric analysis of GITR CD357 expression on human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were cultured for 3 days in medium with Phytohemagglutinin-M PHA-M Stimulated; 1.5 % v/v . The cells were then stained with either Purified Mouse IgG2b, kappa Isotype Control Cat. No. 555740; dashed line histogram or Purified Mouse Anti-Human GITR CD357 antibody Cat. No. 567769; solid line histogram followed by PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . The fluorescent histogram showing GITR CD357 expression or Ig Isotype staining was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FloJo software. Flow cytometric analysis of GITR CD357 expression on human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were cultured for 3 days in medium with Phytohemagglutinin-M PHA-M Stimulated; 1.5 % v/v . The cells were then stained with either Purified Mouse IgG2b, kappa Isotype Control Cat. No. 555740; dashed line histogram or Purified Mouse Anti-Human GITR CD357 antibody Cat. No. 567769; solid line histogram followed by PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . The fluorescent histogram showing GITR CD357 expression or Ig Isotype staining was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FloJo software. Show More Flow cytometric analysis of GITR CD357 expression on human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were cultured for 3 days in medium with Phytohemagglutinin-M PHA-M Stimulated; 1.5 % v/v . The cells were then stained with either Purified Mouse IgG2b, kappa Isotype Control Cat. No. 555740; dashed line histogram or Purified Mouse Anti-Human GITR CD357 antibody Cat. No. 567769; solid line histogram followed by PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . The fluorescent histogram showing GITR CD357 expression or Ig Isotype staining was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FloJo software.