BD PharmingenTM Purified Mouse Anti-Human HLA-ABC

Description

Multiparameter flow cytometric analysis of HLA-ABC expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either Purified Mouse IgG2a, kappa Isotype Control Cat. No. 553454; Left Plot or Purified Mouse Anti-Human HLA-ABC antibody Cat. No. 571698; Right Plot . The cells were washed and then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of HLA-ABC or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo Software. Multiparameter flow cytometric analysis of HLA-ABC expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either Purified Mouse IgG2a, kappa Isotype Control Cat. No. 553454; Left Plot or Purified Mouse Anti-Human HLA-ABC antibody Cat. No. 571698; Right Plot . The cells were washed and then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of HLA-ABC or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo Software. Multiparameter flow cytometric analysis of HLA-ABC expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either Purified Mouse IgG2a, kappa Isotype Control Cat. No. 553454; Left Plot or Purified Mouse Anti-Human HLA-ABC antibody Cat. No. 571698; Right Plot . The cells were washed and then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of HLA-ABC or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of HLA-ABC expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either Purified Mouse IgG2a, kappa Isotype Control Cat. No. 553454; Left Plot or Purified Mouse Anti-Human HLA-ABC antibody Cat. No. 571698; Right Plot . The cells were washed and then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of HLA-ABC or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of HLA-ABC expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either Purified Mouse IgG2a, kappa Isotype Control Cat. No. 553454; Left Plot or Purified Mouse Anti-Human HLA-ABC antibody Cat. No. 571698; Right Plot . The cells were washed and then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of HLA-ABC or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo Software. Multiparameter flow cytometric analysis of HLA-ABC expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either Purified Mouse IgG2a, kappa Isotype Control Cat. No. 553454; Left Plot or Purified Mouse Anti-Human HLA-ABC antibody Cat. No. 571698; Right Plot . The cells were washed and then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of HLA-ABC or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo Software.