BD PharmingenTM Purified Mouse Anti-Human IDO1

Description

Multiparameter flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium with lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with either Purified IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human IDO1 antibody Cat. No. 568020; Right Plot at 0.5 ug/test followed by PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Bivariate pseudocolor plots showing the correlated expression of IDO1 or Ig Isotype control staining versus side light-scatter signals SSC-A were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium with lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with either Purified IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human IDO1 antibody Cat. No. 568020; Right Plot at 0.5 ug/test followed by PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Bivariate pseudocolor plots showing the correlated expression of IDO1 or Ig Isotype control staining versus side light-scatter signals SSC-A were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium with lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with either Purified IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human IDO1 antibody Cat. No. 568020; Right Plot at 0.5 ug/test followed by PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Bivariate pseudocolor plots showing the correlated expression of IDO1 or Ig Isotype control staining versus side light-scatter signals SSC-A were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium with lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with either Purified IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human IDO1 antibody Cat. No. 568020; Right Plot at 0.5 ug/test followed by PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Bivariate pseudocolor plots showing the correlated expression of IDO1 or Ig Isotype control staining versus side light-scatter signals SSC-A were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium with lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with either Purified IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human IDO1 antibody Cat. No. 568020; Right Plot at 0.5 ug/test followed by PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Bivariate pseudocolor plots showing the correlated expression of IDO1 or Ig Isotype control staining versus side light-scatter signals SSC-A were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium with lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with either Purified IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human IDO1 antibody Cat. No. 568020; Right Plot at 0.5 ug/test followed by PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Bivariate pseudocolor plots showing the correlated expression of IDO1 or Ig Isotype control staining versus side light-scatter signals SSC-A were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.