BD PharmingenTM Purified Mouse Anti-Human IL-8

Description

Immunocytochemistry analysis of IL-8 expression on human peripheral blood mononuclear cells. PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37C with LPS Sigma No. L-8274, 1 mug / ml in the presence of GolgiStop Cat. No. 554724 . The activated cells were harvested and the level of IL-8 producing cells was detected by immunocytochemistry using a three-step staining procedure that employs a Biotin Goat anti-mouse IgG secondary antibody Cat. No. 550337 and Streptavdin-horseradish peroxidase Cat. No. 550946 Nomarski optics, original magnification 400X . Immunocytochemistry analysis of IL-8 expression on human peripheral blood mononuclear cells. PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37C with LPS Sigma No. L-8274, 1 mug / ml in the presence of GolgiStop Cat. No. 554724 . The activated cells were harvested and the level of IL-8 producing cells was detected by immunocytochemistry using a three-step staining procedure that employs a Biotin Goat anti-mouse IgG secondary antibody Cat. No. 550337 and Streptavdin-horseradish peroxidase Cat. No. 550946 Nomarski optics, original magnification 400X . Show More Immunocytochemistry analysis of IL-8 expression on human peripheral blood mononuclear cells. PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37C with LPS Sigma No. L-8274, 1 mug / ml in the presence of GolgiStop Cat. No. 554724 . The activated cells were harvested and the level of IL-8 producing cells was detected by immunocytochemistry using a three-step staining procedure that employs a Biotin Goat anti-mouse IgG secondary antibody Cat. No. 550337 and Streptavdin-horseradish peroxidase Cat. No. 550946 Nomarski optics, original magnification 400X .