BD PharmingenTM Purified Mouse Anti-Human Ki-67

Description

Immunofluorescent staining of Ki-67 expressed in Human HeLa cells. Proliferating cells from the HeLa Cervical Adenocarcinoma, ATCC CRM-CCL-2 cell line were cultured, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . Cells were labeled with Purified Mouse Anti-Ki-67 antibody Cat. No. 571599 , washed, and secondarily stained with Alexa Fluor 488 Goat Anti-Mouse Ig pseudocolored green and counter-stained with BD Pharmingen DRAQ7 Cat. No. 564904, pseudocolored red . Images were captured using a standard epifluorescence microscope with a 20x objective. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ki-67 expression in proliferating Human MOLT-4 cells. Proliferating cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , labeled with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Ki-67 antibody Cat. No. 571599; solid line histogram at 0.25 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Histograms showing Ki-67 level or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Immunofluorescent staining of Ki-67 expressed in Human HeLa cells. Proliferating cells from the HeLa Cervical Adenocarcinoma, ATCC CRM-CCL-2 cell line were cultured, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . Cells were labeled with Purified Mouse Anti-Ki-67 antibody Cat. No. 571599 , washed, and secondarily stained with Alexa Fluor 488 Goat Anti-Mouse Ig pseudocolored green and counter-stained with BD Pharmingen DRAQ7 Cat. No. 564904, pseudocolored red . Images were captured using a standard epifluorescence microscope with a 20x objective. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Ki-67 expression in proliferating Human MOLT-4 cells. Proliferating cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , labeled with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Ki-67 antibody Cat. No. 571599; solid line histogram at 0.25 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Histograms showing Ki-67 level or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Immunofluorescent staining of Ki-67 expressed in Human HeLa cells. Proliferating cells from the HeLa Cervical Adenocarcinoma, ATCC CRM-CCL-2 cell line were cultured, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . Cells were labeled with Purified Mouse Anti-Ki-67 antibody Cat. No. 571599 , washed, and secondarily stained with Alexa Fluor 488 Goat Anti-Mouse Ig pseudocolored green and counter-stained with BD Pharmingen DRAQ7 Cat. No. 564904, pseudocolored red . Images were captured using a standard epifluorescence microscope with a 20x objective. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ki-67 expression in proliferating Human MOLT-4 cells. Proliferating cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , labeled with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Ki-67 antibody Cat. No. 571599; solid line histogram at 0.25 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Histograms showing Ki-67 level or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.