BD PharmingenTM Purified Mouse Anti-Human LDLR

Description

Western blot analysis of human LDLR expression. Lysate from 293F cells transfected with human LDLR was prepared for electrophoresis SDS-PAGE in a 2D Tris-Glycine polyacrylamide gel. The proteins were transferred to PVDF membranes and then probed with 0.25, 0.125, 0.06 & 0.03 mug/mL lanes 1-4, respectively of Purified Mouse Anti-Human LDLR. Specific staining was detected with HRP Goat Anti-Mouse Ig Cat No 554002 . Flow cytometric analysis of human LDLR expression. Untransfected dashed-line histogram and human LDLR transfected solid - line histogram 293F cells were washed and stained with Purified Mouse Anti-Human LDLR followed by PE Goat Anti-Mouse Ig Cat. No. 550589 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II cell analyzer. Immunohistochemical staining of LDLR in human hepatic lipidosis top row and hepatic cancer bottom row . Following antigen retrieval with BD Retrievagen A buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded tissue sections were blocked using an Avidin/ Biotin Blocking Kit Vector Laboratories, Cat. No. SP-2001 as recommended by the manufacturer. They were stained with either Purified Mouse IgG2b Isotype Control Cat.No. 557351; Left column or Purified Mouse Anti-Human LDLR antibody Right column . A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. The cell surface and cytoplasm stained positively for the expression of LDLR. Original magnification: 40. Western blot analysis of human LDLR expression. Lysate from 293F cells transfected with human LDLR was prepared for electrophoresis SDS-PAGE in a 2D Tris-Glycine polyacrylamide gel. The proteins were transferred to PVDF membranes and then probed with 0.25, 0.125, 0.06 & 0.03 mug/mL lanes 1-4, respectively of Purified Mouse Anti-Human LDLR. Specific staining was detected with HRP Goat Anti-Mouse Ig Cat No 554002 . Show More Flow cytometric analysis of human LDLR expression. Untransfected dashed-line histogram and human LDLR transfected solid - line histogram 293F cells were washed and stained with Purified Mouse Anti-Human LDLR followed by PE Goat Anti-Mouse Ig Cat. No. 550589 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II cell analyzer. Show More Immunohistochemical staining of LDLR in human hepatic lipidosis top row and hepatic cancer bottom row . Following antigen retrieval with BD Retrievagen A buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded tissue sections were blocked using an Avidin/ Biotin Blocking Kit Vector Laboratories, Cat. No. SP-2001 as recommended by the manufacturer. They were stained with either Purified Mouse IgG2b Isotype Control Cat.No. 557351; Left column or Purified Mouse Anti-Human LDLR antibody Right column . A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. The cell surface and cytoplasm stained positively for the expression of LDLR. Original magnification: 40. Show More Western blot analysis of human LDLR expression. Lysate from 293F cells transfected with human LDLR was prepared for electrophoresis SDS-PAGE in a 2D Tris-Glycine polyacrylamide gel. The proteins were transferred to PVDF membranes and then probed with 0.25, 0.125, 0.06 & 0.03 mug/mL lanes 1-4, respectively of Purified Mouse Anti-Human LDLR. Specific staining was detected with HRP Goat Anti-Mouse Ig Cat No 554002 . Flow cytometric analysis of human LDLR expression. Untransfected dashed-line histogram and human LDLR transfected solid - line histogram 293F cells were washed and stained with Purified Mouse Anti-Human LDLR followed by PE Goat Anti-Mouse Ig Cat. No. 550589 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II cell analyzer. Immunohistochemical staining of LDLR in human hepatic lipidosis top row and hepatic cancer bottom row . Following antigen retrieval with BD Retrievagen A buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded tissue sections were blocked using an Avidin/ Biotin Blocking Kit Vector Laboratories, Cat. No. SP-2001 as recommended by the manufacturer. They were stained with either Purified Mouse IgG2b Isotype Control Cat.No. 557351; Left column or Purified Mouse Anti-Human LDLR antibody Right column . A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. The cell surface and cytoplasm stained positively for the expression of LDLR. Original magnification: 40.