BD PharmingenTM Purified Mouse Anti-Human Perforin

Description

Flow cytometric analysis of perforin expression in human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either Purified Mouse IgG2b, kappa Isotype Control Dashed line histogram; Cat No. 555740/556654 or Purified Mouse Anti-Human Perforin Cat. No 556434 , followed by a FITC conjugated secondary antibody. Flow cytometric analysis was performed using a BD FACScan Flow Cytometer System. Acetone-fixed, frozen tissue section of human lymphoma stained with Purified Mouse Anti-Human Perforin Cat. No. 556434 using a DAB chromogen and Hematoxylin counterstain. Staining is seen in scattered infiltrated lymphocytes, arrows indicate examples. Flow cytometric analysis of perforin expression in human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either Purified Mouse IgG2b, kappa Isotype Control Dashed line histogram; Cat No. 555740/556654 or Purified Mouse Anti-Human Perforin Cat. No 556434 , followed by a FITC conjugated secondary antibody. Flow cytometric analysis was performed using a BD FACScan Flow Cytometer System. Show More Acetone-fixed, frozen tissue section of human lymphoma stained with Purified Mouse Anti-Human Perforin Cat. No. 556434 using a DAB chromogen and Hematoxylin counterstain. Staining is seen in scattered infiltrated lymphocytes, arrows indicate examples. Show More Flow cytometric analysis of perforin expression in human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either Purified Mouse IgG2b, kappa Isotype Control Dashed line histogram; Cat No. 555740/556654 or Purified Mouse Anti-Human Perforin Cat. No 556434 , followed by a FITC conjugated secondary antibody. Flow cytometric analysis was performed using a BD FACScan Flow Cytometer System. Acetone-fixed, frozen tissue section of human lymphoma stained with Purified Mouse Anti-Human Perforin Cat. No. 556434 using a DAB chromogen and Hematoxylin counterstain. Staining is seen in scattered infiltrated lymphocytes, arrows indicate examples.