BD PharmingenTM Purified Mouse Anti-Human RON (CD136)

Description

Analysis of human RON CD136 expression. Left Panel - Flow cytometric analysis of RON CD136 expression on human SW480 cells. Cells from the human SW480 Colorectal adenocarcinoma, ATCC CCL-228 cell line were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Human RON CD136 antibody Cat. No. 565691; solid line histogram followed by staining with PE Goat Anti-Mouse Ig Cat. No. 550589 . The fluorescence histogram showing the expression RON CD136 Ig isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable SW480 cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Right Panel - Immunohistochemical staining of human small intestine. After fixation of small intestine with acetone, sections were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No.550878; Left Image or Purified Mouse Anti-Human Ron CD136 Cat. No. 565691; Right Image . A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. Original magnification: 40. Analysis of human RON CD136 expression. Left Panel - Flow cytometric analysis of RON CD136 expression on human SW480 cells. Cells from the human SW480 Colorectal adenocarcinoma, ATCC CCL-228 cell line were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Human RON CD136 antibody Cat. No. 565691; solid line histogram followed by staining with PE Goat Anti-Mouse Ig Cat. No. 550589 . The fluorescence histogram showing the expression RON CD136 Ig isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable SW480 cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Right Panel - Immunohistochemical staining of human small intestine. After fixation of small intestine with acetone, sections were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No.550878; Left Image or Purified Mouse Anti-Human Ron CD136 Cat. No. 565691; Right Image . A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. Original magnification: 40. Show More Analysis of human RON CD136 expression. Left Panel - Flow cytometric analysis of RON CD136 expression on human SW480 cells. Cells from the human SW480 Colorectal adenocarcinoma, ATCC CCL-228 cell line were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Human RON CD136 antibody Cat. No. 565691; solid line histogram followed by staining with PE Goat Anti-Mouse Ig Cat. No. 550589 . The fluorescence histogram showing the expression RON CD136 Ig isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable SW480 cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Right Panel - Immunohistochemical staining of human small intestine. After fixation of small intestine with acetone, sections were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No.550878; Left Image or Purified Mouse Anti-Human Ron CD136 Cat. No. 565691; Right Image . A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. Original magnification: 40.