BD PharmingenTM Purified Mouse Anti-Human STING

Description

Human STING Expression Panel 1. Western blot analysis of STING expression by THP-1 cells. Proteins from human THP-I Acute monocytic leukemia, ATCC TIB-202 cell lysates were resolved by SDS-PAGE, blotted, and then probed with 0.25 mug/ml Lane 1 , 0.125 mug/ml Lane 2 , and 0.063 mug/ml Lane 3 of Purified Mouse Anti-Human STING antibody Cat. No. 564835 followed by HRP-conjugated Goat Anti-Mouse IgG Cat. No. 554002 , and ECL detection reagents. STING was identified as protein bands of 35-40 kDa. Panel 2. Flow cytometric analysis of STING expression in human peripheral blood leucocytes. Blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. Cells were permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746, Upper Plot or Purified Mouse Anti-Human STING Lower Plot , followed by staining with PE Goat Anti-Mouse Ig Cat. No. 550589 . Contour plots showing the coexpression of STING or Ig Isotype control staining versus side light-scatter characteristics SSC were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Panel 3. Immunohistochemical staining of STING expression in human tonsils. After antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Mouse IgG1 kappa Isotype Control Left Images or Purified Mouse Anti-Human STING Right Images . A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was then used. The Anti-STING antibody stained some lymphocytes and endothelial, epithelial, and dendritic cells. Original magnifications: 20X for Upper and 40X for Lower Images. Human STING Expression Panel 1. Western blot analysis of STING expression by THP-1 cells. Proteins from human THP-I Acute monocytic leukemia, ATCC TIB-202 cell lysates were resolved by SDS-PAGE, blotted, and then probed with 0.25 mug/ml Lane 1 , 0.125 mug/ml Lane 2 , and 0.063 mug/ml Lane 3 of Purified Mouse Anti-Human STING antibody Cat. No. 564835 followed by HRP-conjugated Goat Anti-Mouse IgG Cat. No. 554002 , and ECL detection reagents. STING was identified as protein bands of 35-40 kDa. Panel 2. Flow cytometric analysis of STING expression in human peripheral blood leucocytes. Blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. Cells were permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746, Upper Plot or Purified Mouse Anti-Human STING Lower Plot , followed by staining with PE Goat Anti-Mouse Ig Cat. No. 550589 . Contour plots showing the coexpression of STING or Ig Isotype control staining versus side light-scatter characteristics SSC were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Panel 3. Immunohistochemical staining of STING expression in human tonsils. After antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Mouse IgG1 kappa Isotype Control Left Images or Purified Mouse Anti-Human STING Right Images . A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was then used. The Anti-STING antibody stained some lymphocytes and endothelial, epithelial, and dendritic cells. Original magnifications: 20X for Upper and 40X for Lower Images. Show More Human STING Expression Panel 1. Western blot analysis of STING expression by THP-1 cells. Proteins from human THP-I Acute monocytic leukemia, ATCC TIB-202 cell lysates were resolved by SDS-PAGE, blotted, and then probed with 0.25 mug/ml Lane 1 , 0.125 mug/ml Lane 2 , and 0.063 mug/ml Lane 3 of Purified Mouse Anti-Human STING antibody Cat. No. 564835 followed by HRP-conjugated Goat Anti-Mouse IgG Cat. No. 554002 , and ECL detection reagents. STING was identified as protein bands of 35-40 kDa. Panel 2. Flow cytometric analysis of STING expression in human peripheral blood leucocytes. Blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. Cells were permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746, Upper Plot or Purified Mouse Anti-Human STING Lower Plot , followed by staining with PE Goat Anti-Mouse Ig Cat. No. 550589 . Contour plots showing the coexpression of STING or Ig Isotype control staining versus side light-scatter characteristics SSC were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Panel 3. Immunohistochemical staining of STING expression in human tonsils. After antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Mouse IgG1 kappa Isotype Control Left Images or Purified Mouse Anti-Human STING Right Images . A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was then used. The Anti-STING antibody stained some lymphocytes and endothelial, epithelial, and dendritic cells. Original magnifications: 20X for Upper and 40X for Lower Images.