BD PharmingenTM Purified Mouse Anti-Human Underphosphorylated Retinoblastoma Protein (RB)

Description

Selective binding of G99-549 to underphosphorylated Rb. MOLT-4 cells ATCC CRL-1582, Human T lymphoblast were labeled with 32P -orthophosphate and immunoprecipitated with either clone G99-549 lanes 1 & 3 or clone G3-245 lanes 2 & 4 . G99-549 Cat. No. 554164 recognizes only underphosphorylated Rb whereas G3-245 Cat. No. 554136 recognizes all species of Rb highly phosphorylated, phosphorylated and under phosphorylated . Lanes 1 & 2 - When P32-labeled IP extracts are run on a gel and detected via autoradiography, the radioactive label is greatly reduced in the G99-549 lane 1 compared to the G3-245 lane 2 immunoprecipitates. Lanes 3 & 4 - A western blot of the same gel probing for total Rb Clone G3-245 shows that underphosphorylated Rb is present in the G99-549 immunoprecipitates lane 3 whereas underphosphorylated, phosphorylated, and highly phosphorylated forms are present in the G3-245 immunoprecipitates lane 4 . pRb, underphosphorylated Rb. ppRb, phosphorylated and highly phosphorylated forms of Rb. Selective binding of G99-549 to underphosphorylated Rb. MOLT-4 cells ATCC CRL-1582, Human T lymphoblast were labeled with 32P -orthophosphate and immunoprecipitated with either clone G99-549 lanes 1 & 3 or clone G3-245 lanes 2 & 4 . G99-549 Cat. No. 554164 recognizes only underphosphorylated Rb whereas G3-245 Cat. No. 554136 recognizes all species of Rb highly phosphorylated, phosphorylated and under phosphorylated . Lanes 1 & 2 - When P32-labeled IP extracts are run on a gel and detected via autoradiography, the radioactive label is greatly reduced in the G99-549 lane 1 compared to the G3-245 lane 2 immunoprecipitates. Lanes 3 & 4 - A western blot of the same gel probing for total Rb Clone G3-245 shows that underphosphorylated Rb is present in the G99-549 immunoprecipitates lane 3 whereas underphosphorylated, phosphorylated, and highly phosphorylated forms are present in the G3-245 immunoprecipitates lane 4 . pRb, underphosphorylated Rb. ppRb, phosphorylated and highly phosphorylated forms of Rb. Show More Selective binding of G99-549 to underphosphorylated Rb. MOLT-4 cells ATCC CRL-1582, Human T lymphoblast were labeled with 32P -orthophosphate and immunoprecipitated with either clone G99-549 lanes 1 & 3 or clone G3-245 lanes 2 & 4 . G99-549 Cat. No. 554164 recognizes only underphosphorylated Rb whereas G3-245 Cat. No. 554136 recognizes all species of Rb highly phosphorylated, phosphorylated and under phosphorylated . Lanes 1 & 2 - When P32-labeled IP extracts are run on a gel and detected via autoradiography, the radioactive label is greatly reduced in the G99-549 lane 1 compared to the G3-245 lane 2 immunoprecipitates. Lanes 3 & 4 - A western blot of the same gel probing for total Rb Clone G3-245 shows that underphosphorylated Rb is present in the G99-549 immunoprecipitates lane 3 whereas underphosphorylated, phosphorylated, and highly phosphorylated forms are present in the G3-245 immunoprecipitates lane 4 . pRb, underphosphorylated Rb. ppRb, phosphorylated and highly phosphorylated forms of Rb.