BD PharmingenTM Purified Mouse anti-Islet-1

Description

TOP LEFT: Western Blot analysis of Islet-1 in mouse pancreatic tumor insulinoma . Lysate from Beta-TC-6 cells ATCC CRL-11506 was probed with Purified Mouse anti-Islet-1 monoclonal antibody at titrations of 0.5 lane 1 , 0.25 lane 2 , and 0.125 mug/ml lane 3 . Islet-1 is identified as a band of 45-50 kDa. TOP RIGHT: Immunohistochemical staining of human Islet-1. Following antigen retrieval with BD Retrievagen A buffer Cat. No. 550524 , formalin-fixed paraffin-embedded human pancreas sections were stained with either purified mouse IgG1, kappa Isotype control Cat. No.550878, top panel or Purified Mouse Anti-Human Islet-1 Cat. No.562546, bottom panel . A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig secondary antibody Cat. No. 550337 , Streptavidin HRP Cat. No. 550946 , and DAB substrate kit Cat. No. 550880 was used. As shown in the figure, the lslet-1 staining is mainly nuclear in the pancreas islet. Original magnification: 40X. BOTTOM ROW: Immunofluorescent staining of Islet-1 in spontaneously differentiated human embryonic stem ES cells left panel and mouse pancreatic tumor insulinoma cells right panel . Spontaneously differentiated Emre et al., 2010 H9 human ES cells WiCell, Madison, WI and Beta-T-C6 cells ATCC, CRL-11506 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 , and stained with Purified Mouse anti-Islet-1 monoclonal antibody pseudo-colored green at 1.25 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies . Nuclear staining pseudo-colored blue was either with DAPI left panel or Hoechst 33342 Cat. No. 561908, right panel . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software. TOP LEFT: Western Blot analysis of Islet-1 in mouse pancreatic tumor insulinoma . Lysate from Beta-TC-6 cells ATCC CRL-11506 was probed with Purified Mouse anti-Islet-1 monoclonal antibody at titrations of 0.5 lane 1 , 0.25 lane 2 , and 0.125 mug/ml lane 3 . Islet-1 is identified as a band of 45-50 kDa. TOP RIGHT: Immunohistochemical staining of human Islet-1. Following antigen retrieval with BD Retrievagen A buffer Cat. No. 550524 , formalin-fixed paraffin-embedded human pancreas sections were stained with either purified mouse IgG1, kappa Isotype control Cat. No.550878, top panel or Purified Mouse Anti-Human Islet-1 Cat. No.562546, bottom panel . A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig secondary antibody Cat. No. 550337 , Streptavidin HRP Cat. No. 550946 , and DAB substrate kit Cat. No. 550880 was used. As shown in the figure, the lslet-1 staining is mainly nuclear in the pancreas islet. Original magnification: 40X. BOTTOM ROW: Immunofluorescent staining of Islet-1 in spontaneously differentiated human embryonic stem ES cells left panel and mouse pancreatic tumor insulinoma cells right panel . Spontaneously differentiated Emre et al., 2010 H9 human ES cells WiCell, Madison, WI and Beta-T-C6 cells ATCC, CRL-11506 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 , and stained with Purified Mouse anti-Islet-1 monoclonal antibody pseudo-colored green at 1.25 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies . Nuclear staining pseudo-colored blue was either with DAPI left panel or Hoechst 33342 Cat. No. 561908, right panel . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software. Show More TOP LEFT: Western Blot analysis of Islet-1 in mouse pancreatic tumor insulinoma . Lysate from Beta-TC-6 cells ATCC CRL-11506 was probed with Purified Mouse anti-Islet-1 monoclonal antibody at titrations of 0.5 lane 1 , 0.25 lane 2 , and 0.125 mug/ml lane 3 . Islet-1 is identified as a band of 45-50 kDa. TOP RIGHT: Immunohistochemical staining of human Islet-1. Following antigen retrieval with BD Retrievagen A buffer Cat. No. 550524 , formalin-fixed paraffin-embedded human pancreas sections were stained with either purified mouse IgG1, kappa Isotype control Cat. No.550878, top panel or Purified Mouse Anti-Human Islet-1 Cat. No.562546, bottom panel . A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig secondary antibody Cat. No. 550337 , Streptavidin HRP Cat. No. 550946 , and DAB substrate kit Cat. No. 550880 was used. As shown in the figure, the lslet-1 staining is mainly nuclear in the pancreas islet. Original magnification: 40X. BOTTOM ROW: Immunofluorescent staining of Islet-1 in spontaneously differentiated human embryonic stem ES cells left panel and mouse pancreatic tumor insulinoma cells right panel . Spontaneously differentiated Emre et al., 2010 H9 human ES cells WiCell, Madison, WI and Beta-T-C6 cells ATCC, CRL-11506 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 , and stained with Purified Mouse anti-Islet-1 monoclonal antibody pseudo-colored green at 1.25 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies . Nuclear staining pseudo-colored blue was either with DAPI left panel or Hoechst 33342 Cat. No. 561908, right panel . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software.