BD PharmingenTM Purified Mouse Anti-MDM2

Description

Immunoprecipitation/western blot analysis of MDM2. A MCF7 human breast carcinoma cell lysate was used for immunoprecipitation at 2 ug antibody/1 million cells lanes 1-2 and western blotting at 1 mug/ml lane 1-4 with the anti-MDM2 antibody clone SMP14 ; MCF7 cells lanes 1 and 3 and isotype control lanes 2 and 4 . MDM2 is identified as a band of 95 kDa, as well as a putative MDM2 breakdown product at 62 kDa. The 55 kDa band represents the IgG heavy chain used for immunoprecipitation. Immunofluorescent staining of A549 ATCC CCL-185 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-MDM2 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . The image was taken on a BD Pathway 855 Bioimager using a 20x objective. This antibody also stained HeLa ATCC CCL-2 and U-2 OS ATCC HTB-96 cells and can be used with either perm protocol see Recommended Assay Procedure . Immunoprecipitation/western blot analysis of MDM2. A MCF7 human breast carcinoma cell lysate was used for immunoprecipitation at 2 ug antibody/1 million cells lanes 1-2 and western blotting at 1 mug/ml lane 1-4 with the anti-MDM2 antibody clone SMP14 ; MCF7 cells lanes 1 and 3 and isotype control lanes 2 and 4 . MDM2 is identified as a band of 95 kDa, as well as a putative MDM2 breakdown product at 62 kDa. The 55 kDa band represents the IgG heavy chain used for immunoprecipitation. Show More Immunofluorescent staining of A549 ATCC CCL-185 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-MDM2 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . The image was taken on a BD Pathway 855 Bioimager using a 20x objective. This antibody also stained HeLa ATCC CCL-2 and U-2 OS ATCC HTB-96 cells and can be used with either perm protocol see Recommended Assay Procedure . Show More Immunoprecipitation/western blot analysis of MDM2. A MCF7 human breast carcinoma cell lysate was used for immunoprecipitation at 2 ug antibody/1 million cells lanes 1-2 and western blotting at 1 mug/ml lane 1-4 with the anti-MDM2 antibody clone SMP14 ; MCF7 cells lanes 1 and 3 and isotype control lanes 2 and 4 . MDM2 is identified as a band of 95 kDa, as well as a putative MDM2 breakdown product at 62 kDa. The 55 kDa band represents the IgG heavy chain used for immunoprecipitation. Immunofluorescent staining of A549 ATCC CCL-185 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-MDM2 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . The image was taken on a BD Pathway 855 Bioimager using a 20x objective. This antibody also stained HeLa ATCC CCL-2 and U-2 OS ATCC HTB-96 cells and can be used with either perm protocol see Recommended Assay Procedure .