BD PharmingenTM Purified Mouse anti-Mouse Nanog

Description

Western blot analysis of Nanog expression on mouse embryonic stem cell lysate. Mouse lysate was probed with Purified Mouse anti-Mouse Nanog Cat. No. 560259 at 2.0 lane 1 , 1.0 lane 2 , and 0.5 lane 3 mug/ml, followed by HRP Goat Anti-Mouse Ig Cat. No. 554002 . Nanog is identified as a 42 kDa band. Immunofluorescent staining of mouse embryonic stem ES cell line. ES-E14TG2a cells ATCC CRL-1821 were seeded in a 96-well imaging plate at 10,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with Triton X-100, and stained with Mouse anti-Mouse Nanog antibody according to the Recommended Assay Procedure. The second step reagent was Alexa Fluor 555 goat anti-mouse Ig Invitrogen pseudo colored green . Cell nuclei were counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System using a 20X objective and merged using BD AttoVision software. This antibody also stains F9 cells mouse embryonal carcinoma, ATCC CRL-1720 . It also worked with the Saponin and cold methanol fix/perm protocols see Recommended Assay Procedure , however Saponin permeabilization resulted in higher background staining . Western blot analysis of Nanog expression on mouse embryonic stem cell lysate. Mouse lysate was probed with Purified Mouse anti-Mouse Nanog Cat. No. 560259 at 2.0 lane 1 , 1.0 lane 2 , and 0.5 lane 3 mug/ml, followed by HRP Goat Anti-Mouse Ig Cat. No. 554002 . Nanog is identified as a 42 kDa band. Show More Immunofluorescent staining of mouse embryonic stem ES cell line. ES-E14TG2a cells ATCC CRL-1821 were seeded in a 96-well imaging plate at 10,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with Triton X-100, and stained with Mouse anti-Mouse Nanog antibody according to the Recommended Assay Procedure. The second step reagent was Alexa Fluor 555 goat anti-mouse Ig Invitrogen pseudo colored green . Cell nuclei were counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System using a 20X objective and merged using BD AttoVision software. This antibody also stains F9 cells mouse embryonal carcinoma, ATCC CRL-1720 . It also worked with the Saponin and cold methanol fix/perm protocols see Recommended Assay Procedure , however Saponin permeabilization resulted in higher background staining . Show More Western blot analysis of Nanog expression on mouse embryonic stem cell lysate. Mouse lysate was probed with Purified Mouse anti-Mouse Nanog Cat. No. 560259 at 2.0 lane 1 , 1.0 lane 2 , and 0.5 lane 3 mug/ml, followed by HRP Goat Anti-Mouse Ig Cat. No. 554002 . Nanog is identified as a 42 kDa band. Immunofluorescent staining of mouse embryonic stem ES cell line. ES-E14TG2a cells ATCC CRL-1821 were seeded in a 96-well imaging plate at 10,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with Triton X-100, and stained with Mouse anti-Mouse Nanog antibody according to the Recommended Assay Procedure. The second step reagent was Alexa Fluor 555 goat anti-mouse Ig Invitrogen pseudo colored green . Cell nuclei were counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System using a 20X objective and merged using BD AttoVision software. This antibody also stains F9 cells mouse embryonal carcinoma, ATCC CRL-1720 . It also worked with the Saponin and cold methanol fix/perm protocols see Recommended Assay Procedure , however Saponin permeabilization resulted in higher background staining .