BD PharmingenTM Purified Mouse Anti-Myogenin

Description

LEFT: Western blot analysis of Myogenin. Lysate from human rhabdomyosarcoma cells RH-30 DSMZ, ACC 489 was probed with Purified Mouse Anti-Myogenin monoclonal antibody at 2 mug/ml. Myogenin is identified as a band of 34 kDa. RIGHT: Immunofluorescent analysis of Myogenin expression in rhadbomyosarcoma cells. Human rhabdomyosarcoma cells RH-30 DSMZ, ACC 489 were fixed with BD Cytofix buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100, and stained with Purified Mouse anti-Myogenin monoclonal antibody pseudo-colored green at 0.6 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies , and counter staining was with Hoechst 33342 pseudo-colored blue . The image was captured on a BD Pathway 435 Cell Analyzer and merged using BD AttovisionSoftware. BD Phosflow Perm/Wash Buffer I Cat. No. 557885 and BD Phosflow Perm buffer III Cat. No. 558050 are also suitable for permeabilization for immunofluorescent applications. LEFT: Western blot analysis of Myogenin. Lysate from human rhabdomyosarcoma cells RH-30 DSMZ, ACC 489 was probed with Purified Mouse Anti-Myogenin monoclonal antibody at 2 mug/ml. Myogenin is identified as a band of 34 kDa. RIGHT: Immunofluorescent analysis of Myogenin expression in rhadbomyosarcoma cells. Human rhabdomyosarcoma cells RH-30 DSMZ, ACC 489 were fixed with BD Cytofix buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100, and stained with Purified Mouse anti-Myogenin monoclonal antibody pseudo-colored green at 0.6 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies , and counter staining was with Hoechst 33342 pseudo-colored blue . The image was captured on a BD Pathway 435 Cell Analyzer and merged using BD AttovisionSoftware. BD Phosflow Perm/Wash Buffer I Cat. No. 557885 and BD Phosflow Perm buffer III Cat. No. 558050 are also suitable for permeabilization for immunofluorescent applications. LEFT: Western blot analysis of Myogenin. Lysate from human rhabdomyosarcoma cells RH-30 DSMZ, ACC 489 was probed with Purified Mouse Anti-Myogenin monoclonal antibody at 2 mug/ml. Myogenin is identified as a band of 34 kDa. RIGHT: Immunofluorescent analysis of Myogenin expression in rhadbomyosarcoma cells. Human rhabdomyosarcoma cells RH-30 DSMZ, ACC 489 were fixed with BD Cytofix buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100, and stained with Purified Mouse anti-Myogenin monoclonal antibody pseudo-colored green at 0.6 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies , and counter staining was with Hoechst 33342 pseudo-colored blue . The image was captured on a BD Pathway 435 Cell Analyzer and merged using BD AttovisionSoftware. BD Phosflow Perm/Wash Buffer I Cat. No. 557885 and BD Phosflow Perm buffer III Cat. No. 558050 are also suitable for permeabilization for immunofluorescent applications. Show More LEFT: Western blot analysis of Myogenin. Lysate from human rhabdomyosarcoma cells RH-30 DSMZ, ACC 489 was probed with Purified Mouse Anti-Myogenin monoclonal antibody at 2 mug/ml. Myogenin is identified as a band of 34 kDa. RIGHT: Immunofluorescent analysis of Myogenin expression in rhadbomyosarcoma cells. Human rhabdomyosarcoma cells RH-30 DSMZ, ACC 489 were fixed with BD Cytofix buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100, and stained with Purified Mouse anti-Myogenin monoclonal antibody pseudo-colored green at 0.6 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies , and counter staining was with Hoechst 33342 pseudo-colored blue . The image was captured on a BD Pathway 435 Cell Analyzer and merged using BD AttovisionSoftware. BD Phosflow Perm/Wash Buffer I Cat. No. 557885 and BD Phosflow Perm buffer III Cat. No. 558050 are also suitable for permeabilization for immunofluorescent applications. Show More LEFT: Western blot analysis of Myogenin. Lysate from human rhabdomyosarcoma cells RH-30 DSMZ, ACC 489 was probed with Purified Mouse Anti-Myogenin monoclonal antibody at 2 mug/ml. Myogenin is identified as a band of 34 kDa. RIGHT: Immunofluorescent analysis of Myogenin expression in rhadbomyosarcoma cells. Human rhabdomyosarcoma cells RH-30 DSMZ, ACC 489 were fixed with BD Cytofix buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100, and stained with Purified Mouse anti-Myogenin monoclonal antibody pseudo-colored green at 0.6 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies , and counter staining was with Hoechst 33342 pseudo-colored blue . The image was captured on a BD Pathway 435 Cell Analyzer and merged using BD AttovisionSoftware. BD Phosflow Perm/Wash Buffer I Cat. No. 557885 and BD Phosflow Perm buffer III Cat. No. 558050 are also suitable for permeabilization for immunofluorescent applications. LEFT: Western blot analysis of Myogenin. Lysate from human rhabdomyosarcoma cells RH-30 DSMZ, ACC 489 was probed with Purified Mouse Anti-Myogenin monoclonal antibody at 2 mug/ml. Myogenin is identified as a band of 34 kDa. RIGHT: Immunofluorescent analysis of Myogenin expression in rhadbomyosarcoma cells. Human rhabdomyosarcoma cells RH-30 DSMZ, ACC 489 were fixed with BD Cytofix buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100, and stained with Purified Mouse anti-Myogenin monoclonal antibody pseudo-colored green at 0.6 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies , and counter staining was with Hoechst 33342 pseudo-colored blue . The image was captured on a BD Pathway 435 Cell Analyzer and merged using BD AttovisionSoftware. BD Phosflow Perm/Wash Buffer I Cat. No. 557885 and BD Phosflow Perm buffer III Cat. No. 558050 are also suitable for permeabilization for immunofluorescent applications.