BD PharmingenTM Purified Mouse Anti-Nkx6.1

Description

Western Blot analysis of Nkx6.1 in mouse pancreatic tumor insulinoma cell lysate. Lysate from Beta-T-C6 cells ATCC, CRL-11506 was probed with Purified Mouse Anti-Nkx6.1 monoclonal antibody at titrations of 0.5 lane 1 , 0.25 lane 2 , and 0.125 mug/ml lane 3 . Nkx6.1 is identified as a doublet of approximately 44 kDa. Immunoflourescent staining of Nkx6.1 in mouse pancreatic tumor insulinoma cells. Beta-T-C6 cells ATCC, CRL-11506 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm III Buffer Cat. No. 558050, and stained with Purified Mouse Anti-Nkx6.1 monoclonal antibody pseudo-colored green at 1.2 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies , and counter-staining was with DAPI pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software. Western Blot analysis of Nkx6.1 in mouse pancreatic tumor insulinoma cell lysate. Lysate from Beta-T-C6 cells ATCC, CRL-11506 was probed with Purified Mouse Anti-Nkx6.1 monoclonal antibody at titrations of 0.5 lane 1 , 0.25 lane 2 , and 0.125 mug/ml lane 3 . Nkx6.1 is identified as a doublet of approximately 44 kDa. Show More Immunoflourescent staining of Nkx6.1 in mouse pancreatic tumor insulinoma cells. Beta-T-C6 cells ATCC, CRL-11506 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm III Buffer Cat. No. 558050, and stained with Purified Mouse Anti-Nkx6.1 monoclonal antibody pseudo-colored green at 1.2 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies , and counter-staining was with DAPI pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software. Show More Western Blot analysis of Nkx6.1 in mouse pancreatic tumor insulinoma cell lysate. Lysate from Beta-T-C6 cells ATCC, CRL-11506 was probed with Purified Mouse Anti-Nkx6.1 monoclonal antibody at titrations of 0.5 lane 1 , 0.25 lane 2 , and 0.125 mug/ml lane 3 . Nkx6.1 is identified as a doublet of approximately 44 kDa. Immunoflourescent staining of Nkx6.1 in mouse pancreatic tumor insulinoma cells. Beta-T-C6 cells ATCC, CRL-11506 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm III Buffer Cat. No. 558050, and stained with Purified Mouse Anti-Nkx6.1 monoclonal antibody pseudo-colored green at 1.2 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies , and counter-staining was with DAPI pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software.