BD PharmingenTM Purified Mouse Anti-Nur77

Description

Left Figure: Western blot analysis of Nur77 expression on mouse thymocytes. Thymocytes were stimulated with PMA 20 ng/ml and ionomycin 500 ng/ml at 37C for 2 hr . Lysates were prepared and separated by SDS/PAGE. Blots were probed with Purified Mouse Anti-Nur77 Cat. No. 554088 at concentrations of 2.0 lane 1 , 1.0 lane 2 , and 0.5 mug/ml lane 3 . Visualization was carried out with HRP Goat Anti-Mouse Ig Cat. No. 554002 . Nur77 is detected as a protein of 77 kDa. Right Figure: Flow cytometric analysis of Nur77 expression on stimulated mouse thymocytes . Thymocytes from C57/BL/6 mice were left unstimulated gray line histogram or stimulated black line histograms with PMA 20ng/ml and Ionomycin 500ng/ml for 2 hours and then stained intracellularly with Purified Mouse IgG1, k Isotype Control Cat.No. 554121; black dashed line or Purified Mouse Anti-Nur77 antibody black solid line at 0.125ug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . Purified antibody was followed by PE conjugated Goat Anti-Mouse Ig Multiple Absorption Secondary antibody Cat.No. 550589 . Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Left Figure: Western blot analysis of Nur77 expression on mouse thymocytes. Thymocytes were stimulated with PMA 20 ng/ml and ionomycin 500 ng/ml at 37C for 2 hr . Lysates were prepared and separated by SDS/PAGE. Blots were probed with Purified Mouse Anti-Nur77 Cat. No. 554088 at concentrations of 2.0 lane 1 , 1.0 lane 2 , and 0.5 mug/ml lane 3 . Visualization was carried out with HRP Goat Anti-Mouse Ig Cat. No. 554002 . Nur77 is detected as a protein of 77 kDa. Right Figure: Flow cytometric analysis of Nur77 expression on stimulated mouse thymocytes . Thymocytes from C57/BL/6 mice were left unstimulated gray line histogram or stimulated black line histograms with PMA 20ng/ml and Ionomycin 500ng/ml for 2 hours and then stained intracellularly with Purified Mouse IgG1, k Isotype Control Cat.No. 554121; black dashed line or Purified Mouse Anti-Nur77 antibody black solid line at 0.125ug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . Purified antibody was followed by PE conjugated Goat Anti-Mouse Ig Multiple Absorption Secondary antibody Cat.No. 550589 . Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Left Figure: Western blot analysis of Nur77 expression on mouse thymocytes. Thymocytes were stimulated with PMA 20 ng/ml and ionomycin 500 ng/ml at 37C for 2 hr . Lysates were prepared and separated by SDS/PAGE. Blots were probed with Purified Mouse Anti-Nur77 Cat. No. 554088 at concentrations of 2.0 lane 1 , 1.0 lane 2 , and 0.5 mug/ml lane 3 . Visualization was carried out with HRP Goat Anti-Mouse Ig Cat. No. 554002 . Nur77 is detected as a protein of 77 kDa. Right Figure: Flow cytometric analysis of Nur77 expression on stimulated mouse thymocytes . Thymocytes from C57/BL/6 mice were left unstimulated gray line histogram or stimulated black line histograms with PMA 20ng/ml and Ionomycin 500ng/ml for 2 hours and then stained intracellularly with Purified Mouse IgG1, k Isotype Control Cat.No. 554121; black dashed line or Purified Mouse Anti-Nur77 antibody black solid line at 0.125ug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . Purified antibody was followed by PE conjugated Goat Anti-Mouse Ig Multiple Absorption Secondary antibody Cat.No. 550589 . Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Left Figure: Western blot analysis of Nur77 expression on mouse thymocytes. Thymocytes were stimulated with PMA 20 ng/ml and ionomycin 500 ng/ml at 37C for 2 hr . Lysates were prepared and separated by SDS/PAGE. Blots were probed with Purified Mouse Anti-Nur77 Cat. No. 554088 at concentrations of 2.0 lane 1 , 1.0 lane 2 , and 0.5 mug/ml lane 3 . Visualization was carried out with HRP Goat Anti-Mouse Ig Cat. No. 554002 . Nur77 is detected as a protein of 77 kDa. Right Figure: Flow cytometric analysis of Nur77 expression on stimulated mouse thymocytes . Thymocytes from C57/BL/6 mice were left unstimulated gray line histogram or stimulated black line histograms with PMA 20ng/ml and Ionomycin 500ng/ml for 2 hours and then stained intracellularly with Purified Mouse IgG1, k Isotype Control Cat.No. 554121; black dashed line or Purified Mouse Anti-Nur77 antibody black solid line at 0.125ug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . Purified antibody was followed by PE conjugated Goat Anti-Mouse Ig Multiple Absorption Secondary antibody Cat.No. 550589 . Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Left Figure: Western blot analysis of Nur77 expression on mouse thymocytes. Thymocytes were stimulated with PMA 20 ng/ml and ionomycin 500 ng/ml at 37C for 2 hr . Lysates were prepared and separated by SDS/PAGE. Blots were probed with Purified Mouse Anti-Nur77 Cat. No. 554088 at concentrations of 2.0 lane 1 , 1.0 lane 2 , and 0.5 mug/ml lane 3 . Visualization was carried out with HRP Goat Anti-Mouse Ig Cat. No. 554002 . Nur77 is detected as a protein of 77 kDa. Right Figure: Flow cytometric analysis of Nur77 expression on stimulated mouse thymocytes . Thymocytes from C57/BL/6 mice were left unstimulated gray line histogram or stimulated black line histograms with PMA 20ng/ml and Ionomycin 500ng/ml for 2 hours and then stained intracellularly with Purified Mouse IgG1, k Isotype Control Cat.No. 554121; black dashed line or Purified Mouse Anti-Nur77 antibody black solid line at 0.125ug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . Purified antibody was followed by PE conjugated Goat Anti-Mouse Ig Multiple Absorption Secondary antibody Cat.No. 550589 . Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Left Figure: Western blot analysis of Nur77 expression on mouse thymocytes. Thymocytes were stimulated with PMA 20 ng/ml and ionomycin 500 ng/ml at 37C for 2 hr . Lysates were prepared and separated by SDS/PAGE. Blots were probed with Purified Mouse Anti-Nur77 Cat. No. 554088 at concentrations of 2.0 lane 1 , 1.0 lane 2 , and 0.5 mug/ml lane 3 . Visualization was carried out with HRP Goat Anti-Mouse Ig Cat. No. 554002 . Nur77 is detected as a protein of 77 kDa. Right Figure: Flow cytometric analysis of Nur77 expression on stimulated mouse thymocytes . Thymocytes from C57/BL/6 mice were left unstimulated gray line histogram or stimulated black line histograms with PMA 20ng/ml and Ionomycin 500ng/ml for 2 hours and then stained intracellularly with Purified Mouse IgG1, k Isotype Control Cat.No. 554121; black dashed line or Purified Mouse Anti-Nur77 antibody black solid line at 0.125ug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . Purified antibody was followed by PE conjugated Goat Anti-Mouse Ig Multiple Absorption Secondary antibody Cat.No. 550589 . Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.