BD PharmingenTM Purified Mouse anti-Oct3/4 (Human Isoform A)

Description

Western Blot analysis of Oct3/4 Isoform A in human embryonic stem ES cells. Lysate from H9 human ES cells WiCell,Madison, WI grown in mTESR1 medium StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 15 mug/lane was probed with Purified Mouse anti-Oct3/4 Human Isoform A monoclonal antibody at 0.125 lane 1 , 0.06 lane 2 , and 0.03 lane 3 mug/ml. Oct3/4A is identified as a band of 46 kDa. Immunofluorescent staining of Oct3/4 Isoform A in human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 33 grown on irradiated mouse fibroblasts were fixed with BD Cytofix fixation buffer Cat. No. 554655 , permeabilized, and stained with Purified Mouse anti-Oct3/4 Human Isoform A monoclonal antibody pseudo-colored green at 0.06 mug/mL. The second-step reagent was AlexaFluor 488 goat anti-mouse Ig Life Technologies , and cell nuclei were counter stained with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. The cells were permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 ; 1x BD Perm/Wash Buffer Cat No. 554723 is also suitable for permeabilization. Western Blot analysis of Oct3/4 Isoform A in human embryonic stem ES cells. Lysate from H9 human ES cells WiCell,Madison, WI grown in mTESR1 medium StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 15 mug/lane was probed with Purified Mouse anti-Oct3/4 Human Isoform A monoclonal antibody at 0.125 lane 1 , 0.06 lane 2 , and 0.03 lane 3 mug/ml. Oct3/4A is identified as a band of 46 kDa. Show More Immunofluorescent staining of Oct3/4 Isoform A in human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 33 grown on irradiated mouse fibroblasts were fixed with BD Cytofix fixation buffer Cat. No. 554655 , permeabilized, and stained with Purified Mouse anti-Oct3/4 Human Isoform A monoclonal antibody pseudo-colored green at 0.06 mug/mL. The second-step reagent was AlexaFluor 488 goat anti-mouse Ig Life Technologies , and cell nuclei were counter stained with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. The cells were permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 ; 1x BD Perm/Wash Buffer Cat No. 554723 is also suitable for permeabilization. Show More Western Blot analysis of Oct3/4 Isoform A in human embryonic stem ES cells. Lysate from H9 human ES cells WiCell,Madison, WI grown in mTESR1 medium StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 15 mug/lane was probed with Purified Mouse anti-Oct3/4 Human Isoform A monoclonal antibody at 0.125 lane 1 , 0.06 lane 2 , and 0.03 lane 3 mug/ml. Oct3/4A is identified as a band of 46 kDa. Immunofluorescent staining of Oct3/4 Isoform A in human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 33 grown on irradiated mouse fibroblasts were fixed with BD Cytofix fixation buffer Cat. No. 554655 , permeabilized, and stained with Purified Mouse anti-Oct3/4 Human Isoform A monoclonal antibody pseudo-colored green at 0.06 mug/mL. The second-step reagent was AlexaFluor 488 goat anti-mouse Ig Life Technologies , and cell nuclei were counter stained with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. The cells were permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 ; 1x BD Perm/Wash Buffer Cat No. 554723 is also suitable for permeabilization.