BD PharmingenTM Purified Mouse Anti-Pig CD8b

Description

CD8 expression on peripheral blood lymphocytes. Yorkshire pig whole blood was stained with purified 295/33-25 monoclonal antibody followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 Cat. No. 553390 , then PE-conjugated mAb 74-12-4 anti-pig CD4a, Cat. No. 559586 . Erythrocytes were lysed BD PharmLyse, Cat. No. 555899 , non-viable leukocytes were excluded by staining with 7-AAD BD Via-Probe, Cat. No. 555816/555815 , and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur Flow Cytometry System. CD8 expression on peripheral blood lymphocytes. Yorkshire pig whole blood was stained with purified 295/33-25 monoclonal antibody followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 Cat. No. 553390 , then PE-conjugated mAb 74-12-4 anti-pig CD4a, Cat. No. 559586 . Erythrocytes were lysed BD PharmLyse, Cat. No. 555899 , non-viable leukocytes were excluded by staining with 7-AAD BD Via-Probe, Cat. No. 555816/555815 , and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur Flow Cytometry System. Show More CD8 expression on peripheral blood lymphocytes. Yorkshire pig whole blood was stained with purified 295/33-25 monoclonal antibody followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 Cat. No. 553390 , then PE-conjugated mAb 74-12-4 anti-pig CD4a, Cat. No. 559586 . Erythrocytes were lysed BD PharmLyse, Cat. No. 555899 , non-viable leukocytes were excluded by staining with 7-AAD BD Via-Probe, Cat. No. 555816/555815 , and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur Flow Cytometry System.