BD PharmingenTM Purified Mouse Anti-PTF1A

Description

Analysis of PTF1A expression in rat pancreatic acinar cells by western blot and flow cytometry. Left panel : Lysates of the AR42J cell line ATCC CRL-1492 were electrophoresed SDS-PAGE in a 4-20 % Tris-Glycine polyacrylamide gel, transferred to PVDF membranes and then probed with 2 lane 1 , 1 lane 2 , 0.5 lane 3 , 0.25 lane 4 mg/mL of Purified Mouse anti-PTF1A. Specific staining was detected with HRP Goat Anti-Mouse Ig Cat. No. 554002 . Right panel : AR42J cells were fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 and stained with matching concentrations of either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dashed line or Purified Mouse anti-PTF1A monoclonal antibody solid line . The second-step reagent was PE Goat Anti-Mouse Ig 550589 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact AR42J cells. Flow cytometry was performed using a BD LSRFortessa Flow Cytometer System. Immunohistochemical analysis of PTF1A expression by acinar cells within the human pancreas. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878; left panel or Purified Mouse anti-PTF1A antibody right panel . A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig Cat. No. 550337 , Streptavidin- HRP Cat. No. 550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. The acinar cell nuclei and cytoplasm stained positively for the expression of PTF1A. Original magnification: 40. Analysis of PTF1A expression in rat pancreatic acinar cells by western blot and flow cytometry. Left panel : Lysates of the AR42J cell line ATCC CRL-1492 were electrophoresed SDS-PAGE in a 4-20 % Tris-Glycine polyacrylamide gel, transferred to PVDF membranes and then probed with 2 lane 1 , 1 lane 2 , 0.5 lane 3 , 0.25 lane 4 mg/mL of Purified Mouse anti-PTF1A. Specific staining was detected with HRP Goat Anti-Mouse Ig Cat. No. 554002 . Right panel : AR42J cells were fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 and stained with matching concentrations of either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dashed line or Purified Mouse anti-PTF1A monoclonal antibody solid line . The second-step reagent was PE Goat Anti-Mouse Ig 550589 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact AR42J cells. Flow cytometry was performed using a BD LSRFortessa Flow Cytometer System. Show More Immunohistochemical analysis of PTF1A expression by acinar cells within the human pancreas. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878; left panel or Purified Mouse anti-PTF1A antibody right panel . A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig Cat. No. 550337 , Streptavidin- HRP Cat. No. 550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. The acinar cell nuclei and cytoplasm stained positively for the expression of PTF1A. Original magnification: 40. Show More Analysis of PTF1A expression in rat pancreatic acinar cells by western blot and flow cytometry. Left panel : Lysates of the AR42J cell line ATCC CRL-1492 were electrophoresed SDS-PAGE in a 4-20 % Tris-Glycine polyacrylamide gel, transferred to PVDF membranes and then probed with 2 lane 1 , 1 lane 2 , 0.5 lane 3 , 0.25 lane 4 mg/mL of Purified Mouse anti-PTF1A. Specific staining was detected with HRP Goat Anti-Mouse Ig Cat. No. 554002 . Right panel : AR42J cells were fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 and stained with matching concentrations of either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dashed line or Purified Mouse anti-PTF1A monoclonal antibody solid line . The second-step reagent was PE Goat Anti-Mouse Ig 550589 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact AR42J cells. Flow cytometry was performed using a BD LSRFortessa Flow Cytometer System. Immunohistochemical analysis of PTF1A expression by acinar cells within the human pancreas. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878; left panel or Purified Mouse anti-PTF1A antibody right panel . A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig Cat. No. 550337 , Streptavidin- HRP Cat. No. 550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. The acinar cell nuclei and cytoplasm stained positively for the expression of PTF1A. Original magnification: 40.