BD PharmingenTM Purified Mouse Anti-Rat mGluR1alpha

Description

Immunohistochemical staining of mGluR1alpha on rat brain sections. Formalin fixed paraffin embedded rat brain sections were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 550878; Left Panel or Purified Mouse anti-Rat mGluR1alpha Cat. No 556389; Right Panel . Pretreatment is not needed. Original magnification is 20X. Antibodies were used at 0.5 - 2.0 mug/mL. Development was assisted with the Anti-Mouse Ig HRP Detection Kit Cat. No. 551011 . Western blot analysis of mGluR1alpha expression on rat cerebellum and mouse brain. Rat cerebellum left panel and mouse brain right panel lysates were stained with Purified Mouse Anti-Rat mGluR1alpha at 0.125 lanes 1 and 0.0625 lanes 2 ug/ml for 2 hours at room temperature. Secondary staining was carried out with HRP Goat Anti-Mouse Ig Cat. No. 556389 for 1 hour at room temperature, with an exposure time of 1 second. mGluR1alpha is identified as a 133 kDa band. Immunohistochemical staining of mGluR1alpha on rat brain sections. Formalin fixed paraffin embedded rat brain sections were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 550878; Left Panel or Purified Mouse anti-Rat mGluR1alpha Cat. No 556389; Right Panel . Pretreatment is not needed. Original magnification is 20X. Antibodies were used at 0.5 - 2.0 mug/mL. Development was assisted with the Anti-Mouse Ig HRP Detection Kit Cat. No. 551011 . Show More Western blot analysis of mGluR1alpha expression on rat cerebellum and mouse brain. Rat cerebellum left panel and mouse brain right panel lysates were stained with Purified Mouse Anti-Rat mGluR1alpha at 0.125 lanes 1 and 0.0625 lanes 2 ug/ml for 2 hours at room temperature. Secondary staining was carried out with HRP Goat Anti-Mouse Ig Cat. No. 556389 for 1 hour at room temperature, with an exposure time of 1 second. mGluR1alpha is identified as a 133 kDa band. Show More Immunohistochemical staining of mGluR1alpha on rat brain sections. Formalin fixed paraffin embedded rat brain sections were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 550878; Left Panel or Purified Mouse anti-Rat mGluR1alpha Cat. No 556389; Right Panel . Pretreatment is not needed. Original magnification is 20X. Antibodies were used at 0.5 - 2.0 mug/mL. Development was assisted with the Anti-Mouse Ig HRP Detection Kit Cat. No. 551011 . Western blot analysis of mGluR1alpha expression on rat cerebellum and mouse brain. Rat cerebellum left panel and mouse brain right panel lysates were stained with Purified Mouse Anti-Rat mGluR1alpha at 0.125 lanes 1 and 0.0625 lanes 2 ug/ml for 2 hours at room temperature. Secondary staining was carried out with HRP Goat Anti-Mouse Ig Cat. No. 556389 for 1 hour at room temperature, with an exposure time of 1 second. mGluR1alpha is identified as a 133 kDa band.