BD PharmingenTM Purified Mouse anti-Rb (pS780)

Description

Western blot analysis of Rb pS780 in human embryonic skin cells. Lysates from serum-starved left panel and fetal bovine serum-stimulated right panel WS1 cell line were probed with purified mouse anti-Rb pS780 monoclonal antibody at concentrations of 4.0 lanes 1 and 4 , 2.0 lanes 2 and 5 , and 1.0 mug/ml lanes 3 and 6 . Rb pS780 is identified as a band of 110 kDa in the stimulated cells. Rb pS780 staining on tonsil. Fresh human tonsil, stimulated with in 5 mM Pervanadate solution for 2 hours top row or unstimulated bottom row , was fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the sections were either left untreated left column or treated with a phosphatase to eliminate all phosphorylation right column . The tissue sections were stained with purified Mouse anti-Rb pS780 monoclonal antibody with Hematoxylin counterstaining. Original magnification: 20X. Western blot analysis of Rb pS780 in human embryonic skin cells. Lysates from serum-starved left panel and fetal bovine serum-stimulated right panel WS1 cell line were probed with purified mouse anti-Rb pS780 monoclonal antibody at concentrations of 4.0 lanes 1 and 4 , 2.0 lanes 2 and 5 , and 1.0 mug/ml lanes 3 and 6 . Rb pS780 is identified as a band of 110 kDa in the stimulated cells. Show More Rb pS780 staining on tonsil. Fresh human tonsil, stimulated with in 5 mM Pervanadate solution for 2 hours top row or unstimulated bottom row , was fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the sections were either left untreated left column or treated with a phosphatase to eliminate all phosphorylation right column . The tissue sections were stained with purified Mouse anti-Rb pS780 monoclonal antibody with Hematoxylin counterstaining. Original magnification: 20X. Show More Western blot analysis of Rb pS780 in human embryonic skin cells. Lysates from serum-starved left panel and fetal bovine serum-stimulated right panel WS1 cell line were probed with purified mouse anti-Rb pS780 monoclonal antibody at concentrations of 4.0 lanes 1 and 4 , 2.0 lanes 2 and 5 , and 1.0 mug/ml lanes 3 and 6 . Rb pS780 is identified as a band of 110 kDa in the stimulated cells. Rb pS780 staining on tonsil. Fresh human tonsil, stimulated with in 5 mM Pervanadate solution for 2 hours top row or unstimulated bottom row , was fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the sections were either left untreated left column or treated with a phosphatase to eliminate all phosphorylation right column . The tissue sections were stained with purified Mouse anti-Rb pS780 monoclonal antibody with Hematoxylin counterstaining. Original magnification: 20X.