BD PharmingenTM Purified Mouse Anti-ROR2

Description

Western blot and flow cytometric analyses of ROR2 expression in human chronic myelogenous leukemia cell line. LEFT: Lysate prepared from K562 cells ATCC CCL-243 was electrophoresed SDS-PAGE , transferred to PVDF membranes and probed with 500, 250, 125 and 62.5 ng/mL lanes 1-4, respectively of Purified Mouse Anti-ROR2 Cat. No. 565550 . HRP Goat Anti-Mouse Ig Cat. No. 554002 was used to develop the specific staining. Human ROR2 was identified as a band whose size ranged from 100-150 kDa. RIGHT: K562 cells ATCC CCL-243 were fixed in BD Cytofix fixation buffer Cat. No. 554655 and permeabilized in BD Perm/Wash buffer Cat. No. 554723 . Permeablized cells were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121, dashed line or Purified Mouse Anti-ROR2 Cat. No. 565550, solid line , followed by PE Goat Anti-Mouse Ig Cat. No. 550589 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto II Cell Analyzer. Immunohistochemical staining of ROR2 in human and rat tissue sections. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878; left column or Purified Mouse Anti-ROR2 antibody Cat. No. 565550; right column . A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. Top Row: Human cerebellum. Original magnification: 10 Middle Row: Rat cerebellum. Original magnification: 20 Bottom Row: Rat embryo. Original magnification: 20 Western blot and flow cytometric analyses of ROR2 expression in human chronic myelogenous leukemia cell line. LEFT: Lysate prepared from K562 cells ATCC CCL-243 was electrophoresed SDS-PAGE , transferred to PVDF membranes and probed with 500, 250, 125 and 62.5 ng/mL lanes 1-4, respectively of Purified Mouse Anti-ROR2 Cat. No. 565550 . HRP Goat Anti-Mouse Ig Cat. No. 554002 was used to develop the specific staining. Human ROR2 was identified as a band whose size ranged from 100-150 kDa. RIGHT: K562 cells ATCC CCL-243 were fixed in BD Cytofix fixation buffer Cat. No. 554655 and permeabilized in BD Perm/Wash buffer Cat. No. 554723 . Permeablized cells were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121, dashed line or Purified Mouse Anti-ROR2 Cat. No. 565550, solid line , followed by PE Goat Anti-Mouse Ig Cat. No. 550589 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto II Cell Analyzer. Show More Immunohistochemical staining of ROR2 in human and rat tissue sections. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878; left column or Purified Mouse Anti-ROR2 antibody Cat. No. 565550; right column . A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. Top Row: Human cerebellum. Original magnification: 10 Middle Row: Rat cerebellum. Original magnification: 20 Bottom Row: Rat embryo. Original magnification: 20 Show More Western blot and flow cytometric analyses of ROR2 expression in human chronic myelogenous leukemia cell line. LEFT: Lysate prepared from K562 cells ATCC CCL-243 was electrophoresed SDS-PAGE , transferred to PVDF membranes and probed with 500, 250, 125 and 62.5 ng/mL lanes 1-4, respectively of Purified Mouse Anti-ROR2 Cat. No. 565550 . HRP Goat Anti-Mouse Ig Cat. No. 554002 was used to develop the specific staining. Human ROR2 was identified as a band whose size ranged from 100-150 kDa. RIGHT: K562 cells ATCC CCL-243 were fixed in BD Cytofix fixation buffer Cat. No. 554655 and permeabilized in BD Perm/Wash buffer Cat. No. 554723 . Permeablized cells were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121, dashed line or Purified Mouse Anti-ROR2 Cat. No. 565550, solid line , followed by PE Goat Anti-Mouse Ig Cat. No. 550589 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto II Cell Analyzer. Immunohistochemical staining of ROR2 in human and rat tissue sections. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878; left column or Purified Mouse Anti-ROR2 antibody Cat. No. 565550; right column . A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. Top Row: Human cerebellum. Original magnification: 10 Middle Row: Rat cerebellum. Original magnification: 20 Bottom Row: Rat embryo. Original magnification: 20