BD PharmingenTM Purified Mouse Anti-RUNX3

Description

Western blot and Immunohistochemical analyses of RUNX3 expression. Left Panel: Western blot analysis of RUNX3 expression in Jurkat cell lysate. Lysate was prepared from cells of the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line. Cell lysate proteins were resolved by electrophoresis SDS-PAGE in a 4-20 % Tris-Glycine polyacrylamide gel, transferred to PVDF membranes and then probed with 1 mug/ml of Purified Anti-RUNX3 antibody Cat. No. 564813 followed by HRP-conjugated Goat Anti-Mouse IgG Cat. No. 554002 , and ECL Western blot detection reagents. RUNX3 was identified as protein bands of approximately 40 to 50 kDa. Right Panel: Immunohistochemical staining of RUNX3 expressed in human tonsillar cells. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878; Left Image or Purified Mouse Anti-RUNX3 antibody Cat. No.564813; Right Image . A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. As shown in the Right Image, the RUNX3-specific antibody primarily stained the nuclei of some tonsillar lymphocytes. Original magnification: 40. Western blot and Immunohistochemical analyses of RUNX3 expression. Left Panel: Western blot analysis of RUNX3 expression in Jurkat cell lysate. Lysate was prepared from cells of the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line. Cell lysate proteins were resolved by electrophoresis SDS-PAGE in a 4-20 % Tris-Glycine polyacrylamide gel, transferred to PVDF membranes and then probed with 1 mug/ml of Purified Anti-RUNX3 antibody Cat. No. 564813 followed by HRP-conjugated Goat Anti-Mouse IgG Cat. No. 554002 , and ECL Western blot detection reagents. RUNX3 was identified as protein bands of approximately 40 to 50 kDa. Right Panel: Immunohistochemical staining of RUNX3 expressed in human tonsillar cells. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878; Left Image or Purified Mouse Anti-RUNX3 antibody Cat. No.564813; Right Image . A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. As shown in the Right Image, the RUNX3-specific antibody primarily stained the nuclei of some tonsillar lymphocytes. Original magnification: 40. Show More Western blot and Immunohistochemical analyses of RUNX3 expression. Left Panel: Western blot analysis of RUNX3 expression in Jurkat cell lysate. Lysate was prepared from cells of the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line. Cell lysate proteins were resolved by electrophoresis SDS-PAGE in a 4-20 % Tris-Glycine polyacrylamide gel, transferred to PVDF membranes and then probed with 1 mug/ml of Purified Anti-RUNX3 antibody Cat. No. 564813 followed by HRP-conjugated Goat Anti-Mouse IgG Cat. No. 554002 , and ECL Western blot detection reagents. RUNX3 was identified as protein bands of approximately 40 to 50 kDa. Right Panel: Immunohistochemical staining of RUNX3 expressed in human tonsillar cells. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878; Left Image or Purified Mouse Anti-RUNX3 antibody Cat. No.564813; Right Image . A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin Cat. No. 550337 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. As shown in the Right Image, the RUNX3-specific antibody primarily stained the nuclei of some tonsillar lymphocytes. Original magnification: 40.