BD PharmingenTM Purified Mouse Anti-Sox 9

Description

LEFT: Western blot analysis of Sox9 in human hepatocellular carcinoma cells. After electrophoresis SDS-PAGE of Hep G2 cell lysate Cat. No. 611555 in a 2D Tris-Glycine polyacrylamide gel, the proteins were transferred to PVDF membranes and then probed with Purified Mouse Anti-Sox9 Cat. No. 565492 at concentrations of 0.25, 0.125, and 0.06 mug/ml lanes 1, 2, and 3, respectively . Specific staining was detected with HRP Goat Anti-Mouse Ig Cat No 554002 . Sox9 is identified as a band of 62 kDa. MIDDLE: Flow cytometric analysis of SOX9 expression in human hepatocellular carcinoma cells. Hep G2 cells ATCC HB-8065 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Sox9 solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact Hep G2 cells. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. RIGHT: Immunofluorescent staining of SOX9 in human hepatocellular carcinoma cells. Hep G2 cells ATCC HB-8065 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and stained with Purified Mouse Anti-Human Sox9 pseudo-colored green at 2.5 mug/ml. The second-step reagent was Alexa Fluor 488 Goat Anti-Mouse Ig Life Technologies , and the nuclear counter-staining was with BD Pharmingen DAPI Solution Cat. No. 564907, pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision software. Permeabilization with BD Perm/Wash Cat. No. 554723 is not recommended for use with this antibody. Immunohistochemical staining of Sox9 in human seminal vesicle top row and rat testis bottom row . Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the tissue sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121; left column or Purified Mouse Anti-Sox9 right column . A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig polyclonal secondary antibody Cat. No. 550337 , HRP Streptavidin Cat. No. 550946 and DAB Cat. No. 550880 and Hematoxylin counterstaining were used. Original magnification: 20X top row and 40X bottom row . LEFT: Western blot analysis of Sox9 in human hepatocellular carcinoma cells. After electrophoresis SDS-PAGE of Hep G2 cell lysate Cat. No. 611555 in a 2D Tris-Glycine polyacrylamide gel, the proteins were transferred to PVDF membranes and then probed with Purified Mouse Anti-Sox9 Cat. No. 565492 at concentrations of 0.25, 0.125, and 0.06 mug/ml lanes 1, 2, and 3, respectively . Specific staining was detected with HRP Goat Anti-Mouse Ig Cat No 554002 . Sox9 is identified as a band of 62 kDa. MIDDLE: Flow cytometric analysis of SOX9 expression in human hepatocellular carcinoma cells. Hep G2 cells ATCC HB-8065 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Sox9 solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact Hep G2 cells. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. RIGHT: Immunofluorescent staining of SOX9 in human hepatocellular carcinoma cells. Hep G2 cells ATCC HB-8065 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and stained with Purified Mouse Anti-Human Sox9 pseudo-colored green at 2.5 mug/ml. The second-step reagent was Alexa Fluor 488 Goat Anti-Mouse Ig Life Technologies , and the nuclear counter-staining was with BD Pharmingen DAPI Solution Cat. No. 564907, pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision software. Permeabilization with BD Perm/Wash Cat. No. 554723 is not recommended for use with this antibody. Show More Immunohistochemical staining of Sox9 in human seminal vesicle top row and rat testis bottom row . Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the tissue sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121; left column or Purified Mouse Anti-Sox9 right column . A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig polyclonal secondary antibody Cat. No. 550337 , HRP Streptavidin Cat. No. 550946 and DAB Cat. No. 550880 and Hematoxylin counterstaining were used. Original magnification: 20X top row and 40X bottom row . Show More LEFT: Western blot analysis of Sox9 in human hepatocellular carcinoma cells. After electrophoresis SDS-PAGE of Hep G2 cell lysate Cat. No. 611555 in a 2D Tris-Glycine polyacrylamide gel, the proteins were transferred to PVDF membranes and then probed with Purified Mouse Anti-Sox9 Cat. No. 565492 at concentrations of 0.25, 0.125, and 0.06 mug/ml lanes 1, 2, and 3, respectively . Specific staining was detected with HRP Goat Anti-Mouse Ig Cat No 554002 . Sox9 is identified as a band of 62 kDa. MIDDLE: Flow cytometric analysis of SOX9 expression in human hepatocellular carcinoma cells. Hep G2 cells ATCC HB-8065 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Sox9 solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact Hep G2 cells. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. RIGHT: Immunofluorescent staining of SOX9 in human hepatocellular carcinoma cells. Hep G2 cells ATCC HB-8065 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and stained with Purified Mouse Anti-Human Sox9 pseudo-colored green at 2.5 mug/ml. The second-step reagent was Alexa Fluor 488 Goat Anti-Mouse Ig Life Technologies , and the nuclear counter-staining was with BD Pharmingen DAPI Solution Cat. No. 564907, pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision software. Permeabilization with BD Perm/Wash Cat. No. 554723 is not recommended for use with this antibody. Immunohistochemical staining of Sox9 in human seminal vesicle top row and rat testis bottom row . Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the tissue sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121; left column or Purified Mouse Anti-Sox9 right column . A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig polyclonal secondary antibody Cat. No. 550337 , HRP Streptavidin Cat. No. 550946 and DAB Cat. No. 550880 and Hematoxylin counterstaining were used. Original magnification: 20X top row and 40X bottom row .