BD PharmingenTM Purified Mouse anti-SSEA-1

Description

Western Blot analysis of SSEA-1 in mouse ES cell line. Lysate from ES-E14TG2a cells ATCC CRL-1821 was probed with Purified Mouse anti-SSEA-1 monoclonal antibody Cat. No. 560079 at titrations of 1.0 lane 1 , 0.5 lane 2 , and 0.25 mug/ml lane 3 , followed by HRP Goat Anti-Mouse Ig Cat. No. 554002 . High-molecular-weight molecules bearing the SSEA-1 epitope are identified above 200 kDa. Immunofluorescent staining of mouse ES cell line. ES-E14TG2a cells were cultured, fixed, and stained with Purified Mouse anti-SSEA-1 monoclonal antibody pseudo-colored green according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor 647 goat anti-mouse Ig Invitrogen and counter-staining was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer using a 20X objective and merged using BD Attovision software. Western Blot analysis of SSEA-1 in mouse ES cell line. Lysate from ES-E14TG2a cells ATCC CRL-1821 was probed with Purified Mouse anti-SSEA-1 monoclonal antibody Cat. No. 560079 at titrations of 1.0 lane 1 , 0.5 lane 2 , and 0.25 mug/ml lane 3 , followed by HRP Goat Anti-Mouse Ig Cat. No. 554002 . High-molecular-weight molecules bearing the SSEA-1 epitope are identified above 200 kDa. Show More Immunofluorescent staining of mouse ES cell line. ES-E14TG2a cells were cultured, fixed, and stained with Purified Mouse anti-SSEA-1 monoclonal antibody pseudo-colored green according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor 647 goat anti-mouse Ig Invitrogen and counter-staining was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer using a 20X objective and merged using BD Attovision software. Show More Western Blot analysis of SSEA-1 in mouse ES cell line. Lysate from ES-E14TG2a cells ATCC CRL-1821 was probed with Purified Mouse anti-SSEA-1 monoclonal antibody Cat. No. 560079 at titrations of 1.0 lane 1 , 0.5 lane 2 , and 0.25 mug/ml lane 3 , followed by HRP Goat Anti-Mouse Ig Cat. No. 554002 . High-molecular-weight molecules bearing the SSEA-1 epitope are identified above 200 kDa. Immunofluorescent staining of mouse ES cell line. ES-E14TG2a cells were cultured, fixed, and stained with Purified Mouse anti-SSEA-1 monoclonal antibody pseudo-colored green according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor 647 goat anti-mouse Ig Invitrogen and counter-staining was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer using a 20X objective and merged using BD Attovision software.