BD PharmingenTM Purified Mouse anti-Syk (pY348)

Description

Lysate from control left panel and pervanadate-treated right panel Ramos cells Burkitts lymphoma were probed with mAb I120-722 at concentrations of 0.25 lanes 1 , 0.125 lanes 2 , and 0.0625 lanes 3 mug/ml. Syk pY348 is identified as a strong band of 72 kDa in the pervanadate-treated Ramos cells. Syk pY348 staining on tonsil. Fresh human tonsil, stimulated with a 5 mM Pervanadate solution for 2 hours top row or unstimulated bottom row , was fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the sections were either left untreated left column or treated with a phosphatase to eliminate all phosphorylation right column . The tissue sections were stained with Purified Mouse anti-Syk pY348 monoclonal antibody with Hematoxylin counterstaining. Original magnification: 20X. Lysate from control left panel and pervanadate-treated right panel Ramos cells Burkitts lymphoma were probed with mAb I120-722 at concentrations of 0.25 lanes 1 , 0.125 lanes 2 , and 0.0625 lanes 3 mug/ml. Syk pY348 is identified as a strong band of 72 kDa in the pervanadate-treated Ramos cells. Show More Syk pY348 staining on tonsil. Fresh human tonsil, stimulated with a 5 mM Pervanadate solution for 2 hours top row or unstimulated bottom row , was fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the sections were either left untreated left column or treated with a phosphatase to eliminate all phosphorylation right column . The tissue sections were stained with Purified Mouse anti-Syk pY348 monoclonal antibody with Hematoxylin counterstaining. Original magnification: 20X. Show More Lysate from control left panel and pervanadate-treated right panel Ramos cells Burkitts lymphoma were probed with mAb I120-722 at concentrations of 0.25 lanes 1 , 0.125 lanes 2 , and 0.0625 lanes 3 mug/ml. Syk pY348 is identified as a strong band of 72 kDa in the pervanadate-treated Ramos cells. Syk pY348 staining on tonsil. Fresh human tonsil, stimulated with a 5 mM Pervanadate solution for 2 hours top row or unstimulated bottom row , was fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the sections were either left untreated left column or treated with a phosphatase to eliminate all phosphorylation right column . The tissue sections were stained with Purified Mouse anti-Syk pY348 monoclonal antibody with Hematoxylin counterstaining. Original magnification: 20X.