BD PharmingenTM Purified Mouse anti-TAZ

Description

Western blot analysis of TAZ in transformed human epithelioid carcinoma. HeLa cell lysate Cat. No.611449 was probed with Purified Mouse anti-TAZ monoclonal antibody at concentrations of 0.5, 0.25, and 0.125 mug/ml lanes 1, 2, and 3, respectively . TAZ is identified as a band of 49 kDa. Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were seeded in two 96-well imaging plates Cat. No. 353219 at 10,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with Saponin, and stained with Purified Mouse anti-TAZ pseudocolored green according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor 555 goat anti-mouse Ig Invitrogen . The left image shows TAZ alone, and the right image shows TAZ merged with Hoechst staining. Images were captured on a BD Pathway 435 bioimager using a 20x objective and merged using BD AttoVision software. Other permeabilization methods, cold methanol and Triton X-100, did not work well. Western blot analysis of TAZ in transformed human epithelioid carcinoma. HeLa cell lysate Cat. No.611449 was probed with Purified Mouse anti-TAZ monoclonal antibody at concentrations of 0.5, 0.25, and 0.125 mug/ml lanes 1, 2, and 3, respectively . TAZ is identified as a band of 49 kDa. Show More Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were seeded in two 96-well imaging plates Cat. No. 353219 at 10,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with Saponin, and stained with Purified Mouse anti-TAZ pseudocolored green according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor 555 goat anti-mouse Ig Invitrogen . The left image shows TAZ alone, and the right image shows TAZ merged with Hoechst staining. Images were captured on a BD Pathway 435 bioimager using a 20x objective and merged using BD AttoVision software. Other permeabilization methods, cold methanol and Triton X-100, did not work well. Show More Western blot analysis of TAZ in transformed human epithelioid carcinoma. HeLa cell lysate Cat. No.611449 was probed with Purified Mouse anti-TAZ monoclonal antibody at concentrations of 0.5, 0.25, and 0.125 mug/ml lanes 1, 2, and 3, respectively . TAZ is identified as a band of 49 kDa. Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were seeded in two 96-well imaging plates Cat. No. 353219 at 10,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with Saponin, and stained with Purified Mouse anti-TAZ pseudocolored green according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor 555 goat anti-mouse Ig Invitrogen . The left image shows TAZ alone, and the right image shows TAZ merged with Hoechst staining. Images were captured on a BD Pathway 435 bioimager using a 20x objective and merged using BD AttoVision software. Other permeabilization methods, cold methanol and Triton X-100, did not work well.