BD PharmingenTM Purified NA/LE Mouse Anti-Human CD178

Description

Immunoprecipitation of Fas Ligand FasL . T lymphoma L5187Y cells were transfected with human FasL cDNA and either treated with metalloprotease inhibitor KB8301 or left untreated. KB8301 blocks FasL cleavage resulting in high levels of cell surface FasL. FasL is detected in 35S -labeled cells treated with inhibitor KB8301 lane 1 , but not in untreated cells lane 3 . Isotype negative controls are shown in lanes 2 and 4. Purified NOK-2 Cat. No. 556376 or Mouse IgG2a Cat. No. 553454 was used for immunoprecipitations. Immunoprecipitation of Fas Ligand FasL . T lymphoma L5187Y cells were transfected with human FasL cDNA and either treated with metalloprotease inhibitor KB8301 or left untreated. KB8301 blocks FasL cleavage resulting in high levels of cell surface FasL. FasL is detected in 35S -labeled cells treated with inhibitor KB8301 lane 1 , but not in untreated cells lane 3 . Isotype negative controls are shown in lanes 2 and 4. Purified NOK-2 Cat. No. 556376 or Mouse IgG2a Cat. No. 553454 was used for immunoprecipitations. Show More Immunoprecipitation of Fas Ligand FasL . T lymphoma L5187Y cells were transfected with human FasL cDNA and either treated with metalloprotease inhibitor KB8301 or left untreated. KB8301 blocks FasL cleavage resulting in high levels of cell surface FasL. FasL is detected in 35S -labeled cells treated with inhibitor KB8301 lane 1 , but not in untreated cells lane 3 . Isotype negative controls are shown in lanes 2 and 4. Purified NOK-2 Cat. No. 556376 or Mouse IgG2a Cat. No. 553454 was used for immunoprecipitations.