BD PharmingenTM Purified NA/LE Mouse Anti-Human CD3

Description

CD3 expression and function Left Panel -Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes PBL . Peripheral blood was incubated with either Purified Mouse IgG2a kappa Isotype Control Cat. No. 553454; dashed line histogram or Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 566685; solid line histogram . The cells were washed and stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Panel - Multicolor flow cytometric analysis of CD25 expression on unstimulated and Anti-CD3 plus Anti-CD28 antibody-stimulated human PBL. Human peripheral blood mononuclear cells PBMC were not stimulated Unstimulated: Bottom red-colored histograms or were stimulated 72 h with either soluble Purified NA/LE Mouse Anti-Human CD3 antibody 0.5 mug OKT-3 Ab/ml plus soluble Purified NA/LE Mouse Anti-Human CD28 antibody 5 mug Ab/ml; Cat. No. 555725 Midlevel orange histograms; sol CD3/sol CD28 or plate-bound Mouse Anti-Human CD3 antibody 5 mug OKT-3 Ab/ml overnight coating plus Purified NA/LE Mouse Anti-Human CD28 antibody 5 mug Ab/ml Top blue histograms; pb CD3/sol CD28 as indicated. The cells were stained with BD Horizon BV421 Mouse Anti-Human CD25 Cat. No. 562442/564033 , APC-H7 Mouse Anti Human CD4 Cat. No. 560158/560251 and BD Horizon BUV395 Mouse Anti-Human CD8 Cat. No. 563795/ 563796 antibodies. The fluorescence histograms showing CD25 expression were derived from either CD4+ Left Histograms or CD8+ Right Histograms gated events with the light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. CD3 expression and function Left Panel -Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes PBL . Peripheral blood was incubated with either Purified Mouse IgG2a kappa Isotype Control Cat. No. 553454; dashed line histogram or Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 566685; solid line histogram . The cells were washed and stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Panel - Multicolor flow cytometric analysis of CD25 expression on unstimulated and Anti-CD3 plus Anti-CD28 antibody-stimulated human PBL. Human peripheral blood mononuclear cells PBMC were not stimulated Unstimulated: Bottom red-colored histograms or were stimulated 72 h with either soluble Purified NA/LE Mouse Anti-Human CD3 antibody 0.5 mug OKT-3 Ab/ml plus soluble Purified NA/LE Mouse Anti-Human CD28 antibody 5 mug Ab/ml; Cat. No. 555725 Midlevel orange histograms; sol CD3/sol CD28 or plate-bound Mouse Anti-Human CD3 antibody 5 mug OKT-3 Ab/ml overnight coating plus Purified NA/LE Mouse Anti-Human CD28 antibody 5 mug Ab/ml Top blue histograms; pb CD3/sol CD28 as indicated. The cells were stained with BD Horizon BV421 Mouse Anti-Human CD25 Cat. No. 562442/564033 , APC-H7 Mouse Anti Human CD4 Cat. No. 560158/560251 and BD Horizon BUV395 Mouse Anti-Human CD8 Cat. No. 563795/ 563796 antibodies. The fluorescence histograms showing CD25 expression were derived from either CD4+ Left Histograms or CD8+ Right Histograms gated events with the light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More CD3 expression and function Left Panel -Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes PBL . Peripheral blood was incubated with either Purified Mouse IgG2a kappa Isotype Control Cat. No. 553454; dashed line histogram or Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 566685; solid line histogram . The cells were washed and stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Panel - Multicolor flow cytometric analysis of CD25 expression on unstimulated and Anti-CD3 plus Anti-CD28 antibody-stimulated human PBL. Human peripheral blood mononuclear cells PBMC were not stimulated Unstimulated: Bottom red-colored histograms or were stimulated 72 h with either soluble Purified NA/LE Mouse Anti-Human CD3 antibody 0.5 mug OKT-3 Ab/ml plus soluble Purified NA/LE Mouse Anti-Human CD28 antibody 5 mug Ab/ml; Cat. No. 555725 Midlevel orange histograms; sol CD3/sol CD28 or plate-bound Mouse Anti-Human CD3 antibody 5 mug OKT-3 Ab/ml overnight coating plus Purified NA/LE Mouse Anti-Human CD28 antibody 5 mug Ab/ml Top blue histograms; pb CD3/sol CD28 as indicated. The cells were stained with BD Horizon BV421 Mouse Anti-Human CD25 Cat. No. 562442/564033 , APC-H7 Mouse Anti Human CD4 Cat. No. 560158/560251 and BD Horizon BUV395 Mouse Anti-Human CD8 Cat. No. 563795/ 563796 antibodies. The fluorescence histograms showing CD25 expression were derived from either CD4+ Left Histograms or CD8+ Right Histograms gated events with the light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.