BD PharmingenTM Purified Rabbit Anti- Active Caspase-3

Description

Immunoprecipitation/Western blot analysis of caspase-3 from apoptotic and non-apoptotic cell lysates. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated left panel or were treated with 6 muM camptothecin for 5 hr to induce apoptosis right panel . Immunoprecipitation: Cell lysates were immunoprecipitated with 0.25 - 2 mug/ml of the rabbit anti- active caspase-3 antibody clone C92-605, lanes 1 and 8 2 mug , lanes 2 and 9, 1 mug , lanes 3 and 10 0.5 mug , and lanes 4 and 11 0.25 mug , 1 - 2 mug/ml of a mAb recognizing both pro and active caspase-3 Cat. No. 610322 , lanes 5 and 12 2 mug , lanes 6 and 13 1 mug or 1 mug/ml of a rabbit IgG isotype control Jackson Immunoresearch Cat. No. 011-00000-3 , lanes 7 and 14 . Western blot: Caspase-3 was detected by western blot analysis with an antibody that recognizes both pro- 32 kD and active 20 and 17 kD, reflecting the presence or absence of the caspase-3 pro-domain caspase-3 lanes 1-14 . The results also show that the rabbit anti- active caspase-3 antibody clone C92-605 immunoprecipitated only the active form of caspase-3 lanes 8-11 as compared to the mouse anti-human caspase-3 antibody Cat. No. 610322 which immunoprecipitated both the pro and active forms of caspase-3 lanes 12 and 13 . Bands may be observable at 25 kD and 55-60 kD which represent the light and heavy chains, respectively, of IgG used for the immunoprecipitation. Immunoprecipitation/Western blot analysis of caspase-3 from apoptotic and non-apoptotic cell lysates. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated left panel or were treated with 6 muM camptothecin for 5 hr to induce apoptosis right panel . Immunoprecipitation: Cell lysates were immunoprecipitated with 0.25 - 2 mug/ml of the rabbit anti- active caspase-3 antibody clone C92-605, lanes 1 and 8 2 mug , lanes 2 and 9, 1 mug , lanes 3 and 10 0.5 mug , and lanes 4 and 11 0.25 mug , 1 - 2 mug/ml of a mAb recognizing both pro and active caspase-3 Cat. No. 610322 , lanes 5 and 12 2 mug , lanes 6 and 13 1 mug or 1 mug/ml of a rabbit IgG isotype control Jackson Immunoresearch Cat. No. 011-00000-3 , lanes 7 and 14 . Western blot: Caspase-3 was detected by western blot analysis with an antibody that recognizes both pro- 32 kD and active 20 and 17 kD, reflecting the presence or absence of the caspase-3 pro-domain caspase-3 lanes 1-14 . The results also show that the rabbit anti- active caspase-3 antibody clone C92-605 immunoprecipitated only the active form of caspase-3 lanes 8-11 as compared to the mouse anti-human caspase-3 antibody Cat. No. 610322 which immunoprecipitated both the pro and active forms of caspase-3 lanes 12 and 13 . Bands may be observable at 25 kD and 55-60 kD which represent the light and heavy chains, respectively, of IgG used for the immunoprecipitation. Show More Immunoprecipitation/Western blot analysis of caspase-3 from apoptotic and non-apoptotic cell lysates. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated left panel or were treated with 6 muM camptothecin for 5 hr to induce apoptosis right panel . Immunoprecipitation: Cell lysates were immunoprecipitated with 0.25 - 2 mug/ml of the rabbit anti- active caspase-3 antibody clone C92-605, lanes 1 and 8 2 mug , lanes 2 and 9, 1 mug , lanes 3 and 10 0.5 mug , and lanes 4 and 11 0.25 mug , 1 - 2 mug/ml of a mAb recognizing both pro and active caspase-3 Cat. No. 610322 , lanes 5 and 12 2 mug , lanes 6 and 13 1 mug or 1 mug/ml of a rabbit IgG isotype control Jackson Immunoresearch Cat. No. 011-00000-3 , lanes 7 and 14 . Western blot: Caspase-3 was detected by western blot analysis with an antibody that recognizes both pro- 32 kD and active 20 and 17 kD, reflecting the presence or absence of the caspase-3 pro-domain caspase-3 lanes 1-14 . The results also show that the rabbit anti- active caspase-3 antibody clone C92-605 immunoprecipitated only the active form of caspase-3 lanes 8-11 as compared to the mouse anti-human caspase-3 antibody Cat. No. 610322 which immunoprecipitated both the pro and active forms of caspase-3 lanes 12 and 13 . Bands may be observable at 25 kD and 55-60 kD which represent the light and heavy chains, respectively, of IgG used for the immunoprecipitation.