BD PharmingenTM Purified Rat Anti-Blimp-1

Description

Analyses of Blimp-1 Expression Panel 1. Western blot analysis of Blimp-1 expression in human myeloma cell lines. Lysates from RPMI-8226 ATCC CLL-155; Lane 1 and U266 ATCC TIB-196; Lane 2 cells were electrophoresed, blotted and probed with Purified Rat Anti-Blimp-1 antibody Cat. No. 564703 followed by HRP Goat Anti-Rat Ig Cat. No. 554017 , and ECL detection reagents. The antibody detects Blimp-1 isoforms of 88-92 kDa in both cell lysates and 76-78 kDa in the U266 cell lysate. Note: an unspecific band 250 kDa may be observed upon overexposure of the membranes. Panel 2. Flow cytometric analysis of Blimp-1 expression in a human myeloma cell line. RPMI 8226 cells were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 and stained with either Purified Rat IgG2a, kappa Cat. No. 553927; dashed line histogram or Purified Rat Anti-Blimp-1 antibody solid line histogram followed by staining with PE Goat Anti-Rat Ig Cat. No. 560767 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Panel 3. Immunohistochemical staining of human Blimp-1 in human tonsils. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Rat IgG2a kappa Isotype Control Left Image or Purified Rat Anti-Human Blimp-1 antibody Right Image . A three-step staining procedure that employs Biotin Goat Anti-Rat Immunoglobulin Cat. No. 559286 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. As shown in the Right Image, the Blimp-1 specific antibody primarily stained the nuclei of some Germinal Center cells. Original magnification: 40. Analyses of Blimp-1 Expression Panel 1. Western blot analysis of Blimp-1 expression in human myeloma cell lines. Lysates from RPMI-8226 ATCC CLL-155; Lane 1 and U266 ATCC TIB-196; Lane 2 cells were electrophoresed, blotted and probed with Purified Rat Anti-Blimp-1 antibody Cat. No. 564703 followed by HRP Goat Anti-Rat Ig Cat. No. 554017 , and ECL detection reagents. The antibody detects Blimp-1 isoforms of 88-92 kDa in both cell lysates and 76-78 kDa in the U266 cell lysate. Note: an unspecific band 250 kDa may be observed upon overexposure of the membranes. Panel 2. Flow cytometric analysis of Blimp-1 expression in a human myeloma cell line. RPMI 8226 cells were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 and stained with either Purified Rat IgG2a, kappa Cat. No. 553927; dashed line histogram or Purified Rat Anti-Blimp-1 antibody solid line histogram followed by staining with PE Goat Anti-Rat Ig Cat. No. 560767 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Panel 3. Immunohistochemical staining of human Blimp-1 in human tonsils. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Rat IgG2a kappa Isotype Control Left Image or Purified Rat Anti-Human Blimp-1 antibody Right Image . A three-step staining procedure that employs Biotin Goat Anti-Rat Immunoglobulin Cat. No. 559286 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. As shown in the Right Image, the Blimp-1 specific antibody primarily stained the nuclei of some Germinal Center cells. Original magnification: 40. Show More Analyses of Blimp-1 Expression Panel 1. Western blot analysis of Blimp-1 expression in human myeloma cell lines. Lysates from RPMI-8226 ATCC CLL-155; Lane 1 and U266 ATCC TIB-196; Lane 2 cells were electrophoresed, blotted and probed with Purified Rat Anti-Blimp-1 antibody Cat. No. 564703 followed by HRP Goat Anti-Rat Ig Cat. No. 554017 , and ECL detection reagents. The antibody detects Blimp-1 isoforms of 88-92 kDa in both cell lysates and 76-78 kDa in the U266 cell lysate. Note: an unspecific band 250 kDa may be observed upon overexposure of the membranes. Panel 2. Flow cytometric analysis of Blimp-1 expression in a human myeloma cell line. RPMI 8226 cells were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 and stained with either Purified Rat IgG2a, kappa Cat. No. 553927; dashed line histogram or Purified Rat Anti-Blimp-1 antibody solid line histogram followed by staining with PE Goat Anti-Rat Ig Cat. No. 560767 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Panel 3. Immunohistochemical staining of human Blimp-1 in human tonsils. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Rat IgG2a kappa Isotype Control Left Image or Purified Rat Anti-Human Blimp-1 antibody Right Image . A three-step staining procedure that employs Biotin Goat Anti-Rat Immunoglobulin Cat. No. 559286 , Streptavidin-Horseradish Peroxidase HRP Cat. No.550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. As shown in the Right Image, the Blimp-1 specific antibody primarily stained the nuclei of some Germinal Center cells. Original magnification: 40.