BD PharmingenTM Purified Rat Anti-Human CD120b

Description

Expression of cell surface TNFRII by whole-lysed human blood. Whole human blood was lysed with PharmLyse Cat. No. 555899 prior to staining with hTNFR-M1. Whole lysed human blood was subsequently blocked with normal polyclonal human IgG and stained with purified hTNFR-M1 0.06 mug/10e6 cells, Cat No. 551311 followed by biotinylated goat anti-rat F ab 2 IgG and streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 . Staining with the hTNFR-M1 antibody filled histograms is compared to staining obtained using the isotype control antibody open histograms . The histograms in the figure were derived from gated events with the light scattering characteristics of viable lymphocytes left panel , monocytes center panel and granulocytes right panel . Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Expression of cell surface TNFRII by whole-lysed human blood. Whole human blood was lysed with PharmLyse Cat. No. 555899 prior to staining with hTNFR-M1. Whole lysed human blood was subsequently blocked with normal polyclonal human IgG and stained with purified hTNFR-M1 0.06 mug/10e6 cells, Cat No. 551311 followed by biotinylated goat anti-rat F ab 2 IgG and streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 . Staining with the hTNFR-M1 antibody filled histograms is compared to staining obtained using the isotype control antibody open histograms . The histograms in the figure were derived from gated events with the light scattering characteristics of viable lymphocytes left panel , monocytes center panel and granulocytes right panel . Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Expression of cell surface TNFRII by whole-lysed human blood. Whole human blood was lysed with PharmLyse Cat. No. 555899 prior to staining with hTNFR-M1. Whole lysed human blood was subsequently blocked with normal polyclonal human IgG and stained with purified hTNFR-M1 0.06 mug/10e6 cells, Cat No. 551311 followed by biotinylated goat anti-rat F ab 2 IgG and streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 . Staining with the hTNFR-M1 antibody filled histograms is compared to staining obtained using the isotype control antibody open histograms . The histograms in the figure were derived from gated events with the light scattering characteristics of viable lymphocytes left panel , monocytes center panel and granulocytes right panel . Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Show More Expression of cell surface TNFRII by whole-lysed human blood. Whole human blood was lysed with PharmLyse Cat. No. 555899 prior to staining with hTNFR-M1. Whole lysed human blood was subsequently blocked with normal polyclonal human IgG and stained with purified hTNFR-M1 0.06 mug/10e6 cells, Cat No. 551311 followed by biotinylated goat anti-rat F ab 2 IgG and streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 . Staining with the hTNFR-M1 antibody filled histograms is compared to staining obtained using the isotype control antibody open histograms . The histograms in the figure were derived from gated events with the light scattering characteristics of viable lymphocytes left panel , monocytes center panel and granulocytes right panel . Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Show More Expression of cell surface TNFRII by whole-lysed human blood. Whole human blood was lysed with PharmLyse Cat. No. 555899 prior to staining with hTNFR-M1. Whole lysed human blood was subsequently blocked with normal polyclonal human IgG and stained with purified hTNFR-M1 0.06 mug/10e6 cells, Cat No. 551311 followed by biotinylated goat anti-rat F ab 2 IgG and streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 . Staining with the hTNFR-M1 antibody filled histograms is compared to staining obtained using the isotype control antibody open histograms . The histograms in the figure were derived from gated events with the light scattering characteristics of viable lymphocytes left panel , monocytes center panel and granulocytes right panel . Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Expression of cell surface TNFRII by whole-lysed human blood. Whole human blood was lysed with PharmLyse Cat. No. 555899 prior to staining with hTNFR-M1. Whole lysed human blood was subsequently blocked with normal polyclonal human IgG and stained with purified hTNFR-M1 0.06 mug/10e6 cells, Cat No. 551311 followed by biotinylated goat anti-rat F ab 2 IgG and streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 . Staining with the hTNFR-M1 antibody filled histograms is compared to staining obtained using the isotype control antibody open histograms . The histograms in the figure were derived from gated events with the light scattering characteristics of viable lymphocytes left panel , monocytes center panel and granulocytes right panel . Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.