
551413 ·
BDB Bioscience · Cat: 551413
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Description
Detection of CXCR4 expression on human peripheral lymphocytes by purified 1D9. Human lysed whole blood were stained with 0.25 mug of purified 1D9 using 3-step staining protocol outlined below and anti-human CD4-FITC Cat. No. 555346 The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using purified rat IgG2a Cat. No. 555841 as isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control. The samples were analyzed with a FACScan Flow Cytometer BD Biosciences, San Jose, CA . Detection of CXCR4 expression on human peripheral lymphocytes by purified 1D9. Human lysed whole blood were stained with 0.25 mug of purified 1D9 using 3-step staining protocol outlined below and anti-human CD4-FITC Cat. No. 555346 The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using purified rat IgG2a Cat. No. 555841 as isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control. The samples were analyzed with a FACScan Flow Cytometer BD Biosciences, San Jose, CA . Show More Detection of CXCR4 expression on human peripheral lymphocytes by purified 1D9. Human lysed whole blood were stained with 0.25 mug of purified 1D9 using 3-step staining protocol outlined below and anti-human CD4-FITC Cat. No. 555346 The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using purified rat IgG2a Cat. No. 555841 as isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control. The samples were analyzed with a FACScan Flow Cytometer BD Biosciences, San Jose, CA .