BD PharmingenTM Purified Rat Anti-Human IL-6

Description

PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured with PMA Sigma, Cat. No. P-8139, 5 ng/ml and ionomycin Sigma, Cat. #I-0634, 500 ng/ml in the presence of GolgiStop Cat. No. 554724 overnight at 37C. The activated cells were harvested and the presence of IL-6 producing cells was detected by immunocytochemistry using a three -step staining procedure that employs a Biotin Goat anti-Rat IgG secondary antibody Cat. No. 559286 and a horseradish peroxidase-based detection system. To demonstrate specificity of staining, the binding of Purified Rat Anti-Human IL-6 Cat. No. 559068 was blocked by the preincubation of the purified antibody with excess recombinant human IL-6 protein Cat. No. 550071; data not shown . Nomarski optics, original magnification 400X . PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured with PMA Sigma, Cat. No. P-8139, 5 ng/ml and ionomycin Sigma, Cat. #I-0634, 500 ng/ml in the presence of GolgiStop Cat. No. 554724 overnight at 37C. The activated cells were harvested and the presence of IL-6 producing cells was detected by immunocytochemistry using a three -step staining procedure that employs a Biotin Goat anti-Rat IgG secondary antibody Cat. No. 559286 and a horseradish peroxidase-based detection system. To demonstrate specificity of staining, the binding of Purified Rat Anti-Human IL-6 Cat. No. 559068 was blocked by the preincubation of the purified antibody with excess recombinant human IL-6 protein Cat. No. 550071; data not shown . Nomarski optics, original magnification 400X . Show More PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured with PMA Sigma, Cat. No. P-8139, 5 ng/ml and ionomycin Sigma, Cat. #I-0634, 500 ng/ml in the presence of GolgiStop Cat. No. 554724 overnight at 37C. The activated cells were harvested and the presence of IL-6 producing cells was detected by immunocytochemistry using a three -step staining procedure that employs a Biotin Goat anti-Rat IgG secondary antibody Cat. No. 559286 and a horseradish peroxidase-based detection system. To demonstrate specificity of staining, the binding of Purified Rat Anti-Human IL-6 Cat. No. 559068 was blocked by the preincubation of the purified antibody with excess recombinant human IL-6 protein Cat. No. 550071; data not shown . Nomarski optics, original magnification 400X .