BD PharmingenTM Purified Rat Anti-Mouse C5a

Description

ELISA curves from a sandwich ELISA that measures mouse C5a protein levels. The curves were generated by a sandwich ELISA using the purified I52-1486 antibody as capture antibody, doubling dilutions of recombinant mouse C5a protein as standard and biotinylated I52-278 antibody as detection antibody. Avidin-HRP and the TMB substrate were used to develop the detection stage and mean OD was measured at 450-570 nm. The standard curve is displayed as the concentration of recombinant mouse C5a in ng/ml versus the microwell absorbance in circles . Doubling dilutions of normal mouse serum, starting at 1:2 dilution, are also shown squares . ELISA curves from a sandwich ELISA that measures mouse C5a protein levels. The curves were generated by a sandwich ELISA using the purified I52-1486 antibody as capture antibody, doubling dilutions of recombinant mouse C5a protein as standard and biotinylated I52-278 antibody as detection antibody. Avidin-HRP and the TMB substrate were used to develop the detection stage and mean OD was measured at 450-570 nm. The standard curve is displayed as the concentration of recombinant mouse C5a in ng/ml versus the microwell absorbance in circles . Doubling dilutions of normal mouse serum, starting at 1:2 dilution, are also shown squares . Show More ELISA curves from a sandwich ELISA that measures mouse C5a protein levels. The curves were generated by a sandwich ELISA using the purified I52-1486 antibody as capture antibody, doubling dilutions of recombinant mouse C5a protein as standard and biotinylated I52-278 antibody as detection antibody. Avidin-HRP and the TMB substrate were used to develop the detection stage and mean OD was measured at 450-570 nm. The standard curve is displayed as the concentration of recombinant mouse C5a in ng/ml versus the microwell absorbance in circles . Doubling dilutions of normal mouse serum, starting at 1:2 dilution, are also shown squares .