BD PharmingenTM Purified Rat Anti-Mouse CD195

Description

Expression of mouse CD195 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA 10 mug/ml, final concentration; Sigma . The splenocytes were cultured for additional 5 days in the presence of mouse IL-2 100 U/ml, final concentration; Cat. No. 550069 . The splenocytes were stained with purified rat anti-mouse CD195 antibody C34- 3448 , followed by biotinylated mouse anti-rat IgG2c A92-1, Cat. No. 553909 and Streptavidin- hycoerythrin Cat. No. 554061 following BD Pharmingens staining protocol see Figure, Left panel . The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed using the purified rat IgG2c isotype control purified A23-1; Cat. No. 553982; Right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and isotype control. Expression of mouse CD195 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA 10 mug/ml, final concentration; Sigma . The splenocytes were cultured for additional 5 days in the presence of mouse IL-2 100 U/ml, final concentration; Cat. No. 550069 . The splenocytes were stained with purified rat anti-mouse CD195 antibody C34- 3448 , followed by biotinylated mouse anti-rat IgG2c A92-1, Cat. No. 553909 and Streptavidin- hycoerythrin Cat. No. 554061 following BD Pharmingens staining protocol see Figure, Left panel . The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed using the purified rat IgG2c isotype control purified A23-1; Cat. No. 553982; Right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and isotype control. Expression of mouse CD195 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA 10 mug/ml, final concentration; Sigma . The splenocytes were cultured for additional 5 days in the presence of mouse IL-2 100 U/ml, final concentration; Cat. No. 550069 . The splenocytes were stained with purified rat anti-mouse CD195 antibody C34- 3448 , followed by biotinylated mouse anti-rat IgG2c A92-1, Cat. No. 553909 and Streptavidin- hycoerythrin Cat. No. 554061 following BD Pharmingens staining protocol see Figure, Left panel . The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed using the purified rat IgG2c isotype control purified A23-1; Cat. No. 553982; Right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and isotype control. Show More Expression of mouse CD195 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA 10 mug/ml, final concentration; Sigma . The splenocytes were cultured for additional 5 days in the presence of mouse IL-2 100 U/ml, final concentration; Cat. No. 550069 . The splenocytes were stained with purified rat anti-mouse CD195 antibody C34- 3448 , followed by biotinylated mouse anti-rat IgG2c A92-1, Cat. No. 553909 and Streptavidin- hycoerythrin Cat. No. 554061 following BD Pharmingens staining protocol see Figure, Left panel . The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed using the purified rat IgG2c isotype control purified A23-1; Cat. No. 553982; Right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and isotype control. Show More Expression of mouse CD195 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA 10 mug/ml, final concentration; Sigma . The splenocytes were cultured for additional 5 days in the presence of mouse IL-2 100 U/ml, final concentration; Cat. No. 550069 . The splenocytes were stained with purified rat anti-mouse CD195 antibody C34- 3448 , followed by biotinylated mouse anti-rat IgG2c A92-1, Cat. No. 553909 and Streptavidin- hycoerythrin Cat. No. 554061 following BD Pharmingens staining protocol see Figure, Left panel . The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed using the purified rat IgG2c isotype control purified A23-1; Cat. No. 553982; Right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and isotype control. Expression of mouse CD195 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA 10 mug/ml, final concentration; Sigma . The splenocytes were cultured for additional 5 days in the presence of mouse IL-2 100 U/ml, final concentration; Cat. No. 550069 . The splenocytes were stained with purified rat anti-mouse CD195 antibody C34- 3448 , followed by biotinylated mouse anti-rat IgG2c A92-1, Cat. No. 553909 and Streptavidin- hycoerythrin Cat. No. 554061 following BD Pharmingens staining protocol see Figure, Left panel . The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed using the purified rat IgG2c isotype control purified A23-1; Cat. No. 553982; Right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and isotype control.