BD PharmingenTM Purified Rat Anti-Mouse CD206

Description

Two-color flow cytometric analysis of CD206 expression on Mouse Thioglycolate-elicited Peritoneal Exudate Cells Thio-PECs . C57BL/6 Mouse Thio-PECs were stained with Purified Rat IgG2a, kappa Isotype Control Cat. No. 555841 or Purified Rat Anti-Mouse CD206 antibody Cat. No. 568839 at 1.0 mug/test. The cells were washed and secondarily stained with PE Goat Anti-Rat Ig Cat. No. 550767 . The cells were then blocked with purified normal Rat IgG Rat IgG Isotype Control, Thermo Fisher Cat. No. 10700 before staining with Alexa Fluor 647 Rat Anti-Mouse CD107b antibody Cat. No. 564843 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Thio-PECs. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD206 expression on Mouse Thioglycolate-elicited Peritoneal Exudate Cells Thio-PECs . C57BL/6 Mouse Thio-PECs were stained with Purified Rat IgG2a, kappa Isotype Control Cat. No. 555841 or Purified Rat Anti-Mouse CD206 antibody Cat. No. 568839 at 1.0 mug/test. The cells were washed and secondarily stained with PE Goat Anti-Rat Ig Cat. No. 550767 . The cells were then blocked with purified normal Rat IgG Rat IgG Isotype Control, Thermo Fisher Cat. No. 10700 before staining with Alexa Fluor 647 Rat Anti-Mouse CD107b antibody Cat. No. 564843 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Thio-PECs. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD206 expression on Mouse Thioglycolate-elicited Peritoneal Exudate Cells Thio-PECs . C57BL/6 Mouse Thio-PECs were stained with Purified Rat IgG2a, kappa Isotype Control Cat. No. 555841 or Purified Rat Anti-Mouse CD206 antibody Cat. No. 568839 at 1.0 mug/test. The cells were washed and secondarily stained with PE Goat Anti-Rat Ig Cat. No. 550767 . The cells were then blocked with purified normal Rat IgG Rat IgG Isotype Control, Thermo Fisher Cat. No. 10700 before staining with Alexa Fluor 647 Rat Anti-Mouse CD107b antibody Cat. No. 564843 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Thio-PECs. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD206 expression on Mouse Thioglycolate-elicited Peritoneal Exudate Cells Thio-PECs . C57BL/6 Mouse Thio-PECs were stained with Purified Rat IgG2a, kappa Isotype Control Cat. No. 555841 or Purified Rat Anti-Mouse CD206 antibody Cat. No. 568839 at 1.0 mug/test. The cells were washed and secondarily stained with PE Goat Anti-Rat Ig Cat. No. 550767 . The cells were then blocked with purified normal Rat IgG Rat IgG Isotype Control, Thermo Fisher Cat. No. 10700 before staining with Alexa Fluor 647 Rat Anti-Mouse CD107b antibody Cat. No. 564843 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Thio-PECs. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD206 expression on Mouse Thioglycolate-elicited Peritoneal Exudate Cells Thio-PECs . C57BL/6 Mouse Thio-PECs were stained with Purified Rat IgG2a, kappa Isotype Control Cat. No. 555841 or Purified Rat Anti-Mouse CD206 antibody Cat. No. 568839 at 1.0 mug/test. The cells were washed and secondarily stained with PE Goat Anti-Rat Ig Cat. No. 550767 . The cells were then blocked with purified normal Rat IgG Rat IgG Isotype Control, Thermo Fisher Cat. No. 10700 before staining with Alexa Fluor 647 Rat Anti-Mouse CD107b antibody Cat. No. 564843 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Thio-PECs. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD206 expression on Mouse Thioglycolate-elicited Peritoneal Exudate Cells Thio-PECs . C57BL/6 Mouse Thio-PECs were stained with Purified Rat IgG2a, kappa Isotype Control Cat. No. 555841 or Purified Rat Anti-Mouse CD206 antibody Cat. No. 568839 at 1.0 mug/test. The cells were washed and secondarily stained with PE Goat Anti-Rat Ig Cat. No. 550767 . The cells were then blocked with purified normal Rat IgG Rat IgG Isotype Control, Thermo Fisher Cat. No. 10700 before staining with Alexa Fluor 647 Rat Anti-Mouse CD107b antibody Cat. No. 564843 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Thio-PECs. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.