BD PharmingenTM Purified Rat Anti-Mouse CD324 (E-Cadherin)

Description

Flow cytometric analysis of CD324 E-Cadherin expression on mouse testis embryonal carcinoma cells. F9 cells ATCC CRL-1720 were stained with Purified Rat IgG1, kappa Isotype Control Cat. No. 553922, 555839, or 559072; dashed line histogram or Purified Rat Anti-Mouse CD324 E-Cadherin Cat. No. 567053; solid line histogram at 1 mug/test followed by PE Goat Anti-Rat Ig Cat. No. 550767 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD324 E-Cadherin expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD324 E-Cadherin expression on mouse testis embryonal carcinoma cells. F9 cells ATCC CRL-1720 were stained with Purified Rat IgG1, kappa Isotype Control Cat. No. 553922, 555839, or 559072; dashed line histogram or Purified Rat Anti-Mouse CD324 E-Cadherin Cat. No. 567053; solid line histogram at 1 mug/test followed by PE Goat Anti-Rat Ig Cat. No. 550767 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD324 E-Cadherin expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD324 E-Cadherin expression on mouse testis embryonal carcinoma cells. F9 cells ATCC CRL-1720 were stained with Purified Rat IgG1, kappa Isotype Control Cat. No. 553922, 555839, or 559072; dashed line histogram or Purified Rat Anti-Mouse CD324 E-Cadherin Cat. No. 567053; solid line histogram at 1 mug/test followed by PE Goat Anti-Rat Ig Cat. No. 550767 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD324 E-Cadherin expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.