BD PharmingenTM Purified Rat Anti-Mouse CD34

Description

Identification of CD34+ and CD34- subpopulations of hematopoietic progenitors. BALB/C mouse bone marrow cells were incubated with either purified monoclonal antibody RAM34 Cat. No. 553731, right panel or purified rat IgG2a, kappa isotype control clone R35-95, Cat. No. 553927, left panel , followed by second step staining with FITC polyclonal goat-anti-rat Ig Cat. No. 554016 . Cells were washed and blocked with mouse Fc Block purified anti-CD16/CD32mAb Cat. No. 553141/553142 and normal rat serum, followed by staining with APC Lineage Cocktail Cat. No. 558074 to identify major lineage committed cells. The contour plots were derived from the gated events based on light scattering characteristics of leukocytes and fluorescence characteristics of the CD34 and Lineage markers . Flow cytometry was performed on a BD FACSCanto System. Immunohistochemistry application of CD34 antibody. Immunohistochemistry staining of anti-mouse CD34 antibody clone RAM34 on formalin fixed paraffin embedded mouse embryonic day 11 tissue section with citrate buffer for antigen retrieval. The RAM34 antibody stains on endothelial cells of vessels and blood stem cells in vessels lower panel with isotype control upper panel . The secondary antibody was used biotin polyclonal goat anti-rat IgG Cat. No. 559286 and followed by Streptavidin -HRP Cat. No. 550946 , and DAB detection kit Cat. No. 550880 . Original amplification 40X. Identification of CD34+ and CD34- subpopulations of hematopoietic progenitors. BALB/C mouse bone marrow cells were incubated with either purified monoclonal antibody RAM34 Cat. No. 553731, right panel or purified rat IgG2a, kappa isotype control clone R35-95, Cat. No. 553927, left panel , followed by second step staining with FITC polyclonal goat-anti-rat Ig Cat. No. 554016 . Cells were washed and blocked with mouse Fc Block purified anti-CD16/CD32mAb Cat. No. 553141/553142 and normal rat serum, followed by staining with APC Lineage Cocktail Cat. No. 558074 to identify major lineage committed cells. The contour plots were derived from the gated events based on light scattering characteristics of leukocytes and fluorescence characteristics of the CD34 and Lineage markers . Flow cytometry was performed on a BD FACSCanto System. Show More Immunohistochemistry application of CD34 antibody. Immunohistochemistry staining of anti-mouse CD34 antibody clone RAM34 on formalin fixed paraffin embedded mouse embryonic day 11 tissue section with citrate buffer for antigen retrieval. The RAM34 antibody stains on endothelial cells of vessels and blood stem cells in vessels lower panel with isotype control upper panel . The secondary antibody was used biotin polyclonal goat anti-rat IgG Cat. No. 559286 and followed by Streptavidin -HRP Cat. No. 550946 , and DAB detection kit Cat. No. 550880 . Original amplification 40X. Show More Identification of CD34+ and CD34- subpopulations of hematopoietic progenitors. BALB/C mouse bone marrow cells were incubated with either purified monoclonal antibody RAM34 Cat. No. 553731, right panel or purified rat IgG2a, kappa isotype control clone R35-95, Cat. No. 553927, left panel , followed by second step staining with FITC polyclonal goat-anti-rat Ig Cat. No. 554016 . Cells were washed and blocked with mouse Fc Block purified anti-CD16/CD32mAb Cat. No. 553141/553142 and normal rat serum, followed by staining with APC Lineage Cocktail Cat. No. 558074 to identify major lineage committed cells. The contour plots were derived from the gated events based on light scattering characteristics of leukocytes and fluorescence characteristics of the CD34 and Lineage markers . Flow cytometry was performed on a BD FACSCanto System. Immunohistochemistry application of CD34 antibody. Immunohistochemistry staining of anti-mouse CD34 antibody clone RAM34 on formalin fixed paraffin embedded mouse embryonic day 11 tissue section with citrate buffer for antigen retrieval. The RAM34 antibody stains on endothelial cells of vessels and blood stem cells in vessels lower panel with isotype control upper panel . The secondary antibody was used biotin polyclonal goat anti-rat IgG Cat. No. 559286 and followed by Streptavidin -HRP Cat. No. 550946 , and DAB detection kit Cat. No. 550880 . Original amplification 40X.