BD PharmingenTM Purified Rat Anti-Mouse CD63

Description

Analyses of CD63 expression. Panel 1 and 2. Multicolor flow cytometric analysis of CD63 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with either Purified Rat IgG2a, kappa Isotype Control Cat. No. 553927; Panel 1 or Purified Rat Anti-Mouse CD63 antibody Cat. No. 564221; Panel 2 . The cells were washed and counterstained with PE Goat Anti-Rat Ig Cat. No. 550767 . After washing, the cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 553129/561083 . Two-color flow cytometric dot plots show the correlated expression patterns of Ly-6G and Ly-6C versus CD63 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Panel 3. Immunofluorescent staining of CD63 in bEnd.3 cells. Adherent bEnd.3 cells ATCC CRL-2299 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100 Sigma, X100 , and stained with either Purified Rat IgG2a, kappa Isotype Control Upper Panel 3 or Purified Rat Anti-Mouse CD63 antibody Lower Panel 3 . The cells were washed and then counterstained with Alexa Fluor 488 Goat Anti-Rat IgG H+L Antibody Life Technologies, Cat. No. A11006; pseudocolored green and Hoechst 33342 Solution Cat. No. 561908; pseudocolored blue . Images were analyzed using a BD Pathway 435 cell imager and merged using BD AttoVision Software. Analyses of CD63 expression. Panel 1 and 2. Multicolor flow cytometric analysis of CD63 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with either Purified Rat IgG2a, kappa Isotype Control Cat. No. 553927; Panel 1 or Purified Rat Anti-Mouse CD63 antibody Cat. No. 564221; Panel 2 . The cells were washed and counterstained with PE Goat Anti-Rat Ig Cat. No. 550767 . After washing, the cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 553129/561083 . Two-color flow cytometric dot plots show the correlated expression patterns of Ly-6G and Ly-6C versus CD63 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Panel 3. Immunofluorescent staining of CD63 in bEnd.3 cells. Adherent bEnd.3 cells ATCC CRL-2299 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100 Sigma, X100 , and stained with either Purified Rat IgG2a, kappa Isotype Control Upper Panel 3 or Purified Rat Anti-Mouse CD63 antibody Lower Panel 3 . The cells were washed and then counterstained with Alexa Fluor 488 Goat Anti-Rat IgG H+L Antibody Life Technologies, Cat. No. A11006; pseudocolored green and Hoechst 33342 Solution Cat. No. 561908; pseudocolored blue . Images were analyzed using a BD Pathway 435 cell imager and merged using BD AttoVision Software. Show More Analyses of CD63 expression. Panel 1 and 2. Multicolor flow cytometric analysis of CD63 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with either Purified Rat IgG2a, kappa Isotype Control Cat. No. 553927; Panel 1 or Purified Rat Anti-Mouse CD63 antibody Cat. No. 564221; Panel 2 . The cells were washed and counterstained with PE Goat Anti-Rat Ig Cat. No. 550767 . After washing, the cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 553129/561083 . Two-color flow cytometric dot plots show the correlated expression patterns of Ly-6G and Ly-6C versus CD63 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Panel 3. Immunofluorescent staining of CD63 in bEnd.3 cells. Adherent bEnd.3 cells ATCC CRL-2299 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100 Sigma, X100 , and stained with either Purified Rat IgG2a, kappa Isotype Control Upper Panel 3 or Purified Rat Anti-Mouse CD63 antibody Lower Panel 3 . The cells were washed and then counterstained with Alexa Fluor 488 Goat Anti-Rat IgG H+L Antibody Life Technologies, Cat. No. A11006; pseudocolored green and Hoechst 33342 Solution Cat. No. 561908; pseudocolored blue . Images were analyzed using a BD Pathway 435 cell imager and merged using BD AttoVision Software.