BD PharmingenTM Purified Rat Anti-Mouse CD86

Description

Upregulation of membrane CD86 B7-2 on activated B lymphocytes. Freshly isolated left panel or 72-hour LPS-stimulated BALB/c splenocytes right panel were pretreated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142 and either unstained shaded histograms or stained with purified GL1 mAb open histograms followed by FITC-conjugated anti-rat IgG2a mAb RG7/1.30 Cat. No. 553896, shaded and open histograms . Flow cytometry was performed on a BD FACScan Flow Cytometry System. Resting lymphocytes left panel or activated blasts right panel were selected according to light-scatter profile. Upregulation of membrane CD86 B7-2 on activated B lymphocytes. Freshly isolated left panel or 72-hour LPS-stimulated BALB/c splenocytes right panel were pretreated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142 and either unstained shaded histograms or stained with purified GL1 mAb open histograms followed by FITC-conjugated anti-rat IgG2a mAb RG7/1.30 Cat. No. 553896, shaded and open histograms . Flow cytometry was performed on a BD FACScan Flow Cytometry System. Resting lymphocytes left panel or activated blasts right panel were selected according to light-scatter profile. Upregulation of membrane CD86 B7-2 on activated B lymphocytes. Freshly isolated left panel or 72-hour LPS-stimulated BALB/c splenocytes right panel were pretreated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142 and either unstained shaded histograms or stained with purified GL1 mAb open histograms followed by FITC-conjugated anti-rat IgG2a mAb RG7/1.30 Cat. No. 553896, shaded and open histograms . Flow cytometry was performed on a BD FACScan Flow Cytometry System. Resting lymphocytes left panel or activated blasts right panel were selected according to light-scatter profile. Show More Upregulation of membrane CD86 B7-2 on activated B lymphocytes. Freshly isolated left panel or 72-hour LPS-stimulated BALB/c splenocytes right panel were pretreated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142 and either unstained shaded histograms or stained with purified GL1 mAb open histograms followed by FITC-conjugated anti-rat IgG2a mAb RG7/1.30 Cat. No. 553896, shaded and open histograms . Flow cytometry was performed on a BD FACScan Flow Cytometry System. Resting lymphocytes left panel or activated blasts right panel were selected according to light-scatter profile. Show More Upregulation of membrane CD86 B7-2 on activated B lymphocytes. Freshly isolated left panel or 72-hour LPS-stimulated BALB/c splenocytes right panel were pretreated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142 and either unstained shaded histograms or stained with purified GL1 mAb open histograms followed by FITC-conjugated anti-rat IgG2a mAb RG7/1.30 Cat. No. 553896, shaded and open histograms . Flow cytometry was performed on a BD FACScan Flow Cytometry System. Resting lymphocytes left panel or activated blasts right panel were selected according to light-scatter profile. Upregulation of membrane CD86 B7-2 on activated B lymphocytes. Freshly isolated left panel or 72-hour LPS-stimulated BALB/c splenocytes right panel were pretreated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142 and either unstained shaded histograms or stained with purified GL1 mAb open histograms followed by FITC-conjugated anti-rat IgG2a mAb RG7/1.30 Cat. No. 553896, shaded and open histograms . Flow cytometry was performed on a BD FACScan Flow Cytometry System. Resting lymphocytes left panel or activated blasts right panel were selected according to light-scatter profile.