BD PharmingenTM Purified Rat Anti-Mouse H2-M

Description

Two-color analysis of the cytoplasmic expression of H2-M in splenic B lymphocytes and dendritic cells. Fixed and permeabilized C57BL/6 splenocytes were stained with either purified rat IgG1, kappa isotype control mAb R3-34 Cat. No. 553922, top panels or purified mAb 2E5A bottom panels in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4 G2, Cat. No. 553141/553142 , followed by FITC-conjugated anti-rat IgG1 mAb RG11/39.4 Cat. No. 553892 . B lymphocytes were identified by staining with PE-conjugated anti-mouse CD19 mAb 1D3 Cat. No. 557399/553786, left panels , and dendritic cells were identified with PE-conjugated anti-mouse CD11c mAb HL3 Cat. No. 557401/553802, right panels . Flow cytometry was performed on a BD FACSCalibur System BD Biosciences, San Jose, CA . Two-color analysis of the cytoplasmic expression of H2-M in splenic B lymphocytes and dendritic cells. Fixed and permeabilized C57BL/6 splenocytes were stained with either purified rat IgG1, kappa isotype control mAb R3-34 Cat. No. 553922, top panels or purified mAb 2E5A bottom panels in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4 G2, Cat. No. 553141/553142 , followed by FITC-conjugated anti-rat IgG1 mAb RG11/39.4 Cat. No. 553892 . B lymphocytes were identified by staining with PE-conjugated anti-mouse CD19 mAb 1D3 Cat. No. 557399/553786, left panels , and dendritic cells were identified with PE-conjugated anti-mouse CD11c mAb HL3 Cat. No. 557401/553802, right panels . Flow cytometry was performed on a BD FACSCalibur System BD Biosciences, San Jose, CA . Show More Two-color analysis of the cytoplasmic expression of H2-M in splenic B lymphocytes and dendritic cells. Fixed and permeabilized C57BL/6 splenocytes were stained with either purified rat IgG1, kappa isotype control mAb R3-34 Cat. No. 553922, top panels or purified mAb 2E5A bottom panels in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4 G2, Cat. No. 553141/553142 , followed by FITC-conjugated anti-rat IgG1 mAb RG11/39.4 Cat. No. 553892 . B lymphocytes were identified by staining with PE-conjugated anti-mouse CD19 mAb 1D3 Cat. No. 557399/553786, left panels , and dendritic cells were identified with PE-conjugated anti-mouse CD11c mAb HL3 Cat. No. 557401/553802, right panels . Flow cytometry was performed on a BD FACSCalibur System BD Biosciences, San Jose, CA .