BD PharmingenTM Purified Rat Anti-Mouse IL-2

Description

Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/c mice were stimulated for 5 hours with hamster anti-mouse CD3 2 mug/ml final concentration; clone 145-2C11, Cat. No. 553057 and hamster anti-mouse CD28 2 mug/ml final concentration; clone 37.51, Cat. No. 553294 antibodies in the presence of BD GolgiStop 3 muM final concentration; Cat. No. 554724 . The cells were harvested, stained with 0.06 mug of PE-conjugated rat anti-mouse CD4 PE-RM4-5, Cat. No. 553049 , fixed, permeabilized, and subsequently stained with 0.06 mug of FITC-conjugated rat anti-mouse IL-2 antibody FITC-JES6-5H4, Cat. No. 554427, left panel . To demonstrate specificity of staining, the binding by the FITC-JES6-5H4 antibody was blocked by preincubation of the conjugated antibody with recombinant mouse IL-2 0.12 mug, Cat. No. 550069; middle panel , and by preincubation of the fixed/permeabilized cells with unlabeled JES6-5H4 antibody 2.5 mug; Cat. No. 554425; right panel prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls. Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/c mice were stimulated for 5 hours with hamster anti-mouse CD3 2 mug/ml final concentration; clone 145-2C11, Cat. No. 553057 and hamster anti-mouse CD28 2 mug/ml final concentration; clone 37.51, Cat. No. 553294 antibodies in the presence of BD GolgiStop 3 muM final concentration; Cat. No. 554724 . The cells were harvested, stained with 0.06 mug of PE-conjugated rat anti-mouse CD4 PE-RM4-5, Cat. No. 553049 , fixed, permeabilized, and subsequently stained with 0.06 mug of FITC-conjugated rat anti-mouse IL-2 antibody FITC-JES6-5H4, Cat. No. 554427, left panel . To demonstrate specificity of staining, the binding by the FITC-JES6-5H4 antibody was blocked by preincubation of the conjugated antibody with recombinant mouse IL-2 0.12 mug, Cat. No. 550069; middle panel , and by preincubation of the fixed/permeabilized cells with unlabeled JES6-5H4 antibody 2.5 mug; Cat. No. 554425; right panel prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls. Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/c mice were stimulated for 5 hours with hamster anti-mouse CD3 2 mug/ml final concentration; clone 145-2C11, Cat. No. 553057 and hamster anti-mouse CD28 2 mug/ml final concentration; clone 37.51, Cat. No. 553294 antibodies in the presence of BD GolgiStop 3 muM final concentration; Cat. No. 554724 . The cells were harvested, stained with 0.06 mug of PE-conjugated rat anti-mouse CD4 PE-RM4-5, Cat. No. 553049 , fixed, permeabilized, and subsequently stained with 0.06 mug of FITC-conjugated rat anti-mouse IL-2 antibody FITC-JES6-5H4, Cat. No. 554427, left panel . To demonstrate specificity of staining, the binding by the FITC-JES6-5H4 antibody was blocked by preincubation of the conjugated antibody with recombinant mouse IL-2 0.12 mug, Cat. No. 550069; middle panel , and by preincubation of the fixed/permeabilized cells with unlabeled JES6-5H4 antibody 2.5 mug; Cat. No. 554425; right panel prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls. Show More Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/c mice were stimulated for 5 hours with hamster anti-mouse CD3 2 mug/ml final concentration; clone 145-2C11, Cat. No. 553057 and hamster anti-mouse CD28 2 mug/ml final concentration; clone 37.51, Cat. No. 553294 antibodies in the presence of BD GolgiStop 3 muM final concentration; Cat. No. 554724 . The cells were harvested, stained with 0.06 mug of PE-conjugated rat anti-mouse CD4 PE-RM4-5, Cat. No. 553049 , fixed, permeabilized, and subsequently stained with 0.06 mug of FITC-conjugated rat anti-mouse IL-2 antibody FITC-JES6-5H4, Cat. No. 554427, left panel . To demonstrate specificity of staining, the binding by the FITC-JES6-5H4 antibody was blocked by preincubation of the conjugated antibody with recombinant mouse IL-2 0.12 mug, Cat. No. 550069; middle panel , and by preincubation of the fixed/permeabilized cells with unlabeled JES6-5H4 antibody 2.5 mug; Cat. No. 554425; right panel prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls. Show More Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/c mice were stimulated for 5 hours with hamster anti-mouse CD3 2 mug/ml final concentration; clone 145-2C11, Cat. No. 553057 and hamster anti-mouse CD28 2 mug/ml final concentration; clone 37.51, Cat. No. 553294 antibodies in the presence of BD GolgiStop 3 muM final concentration; Cat. No. 554724 . The cells were harvested, stained with 0.06 mug of PE-conjugated rat anti-mouse CD4 PE-RM4-5, Cat. No. 553049 , fixed, permeabilized, and subsequently stained with 0.06 mug of FITC-conjugated rat anti-mouse IL-2 antibody FITC-JES6-5H4, Cat. No. 554427, left panel . To demonstrate specificity of staining, the binding by the FITC-JES6-5H4 antibody was blocked by preincubation of the conjugated antibody with recombinant mouse IL-2 0.12 mug, Cat. No. 550069; middle panel , and by preincubation of the fixed/permeabilized cells with unlabeled JES6-5H4 antibody 2.5 mug; Cat. No. 554425; right panel prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls. Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/c mice were stimulated for 5 hours with hamster anti-mouse CD3 2 mug/ml final concentration; clone 145-2C11, Cat. No. 553057 and hamster anti-mouse CD28 2 mug/ml final concentration; clone 37.51, Cat. No. 553294 antibodies in the presence of BD GolgiStop 3 muM final concentration; Cat. No. 554724 . The cells were harvested, stained with 0.06 mug of PE-conjugated rat anti-mouse CD4 PE-RM4-5, Cat. No. 553049 , fixed, permeabilized, and subsequently stained with 0.06 mug of FITC-conjugated rat anti-mouse IL-2 antibody FITC-JES6-5H4, Cat. No. 554427, left panel . To demonstrate specificity of staining, the binding by the FITC-JES6-5H4 antibody was blocked by preincubation of the conjugated antibody with recombinant mouse IL-2 0.12 mug, Cat. No. 550069; middle panel , and by preincubation of the fixed/permeabilized cells with unlabeled JES6-5H4 antibody 2.5 mug; Cat. No. 554425; right panel prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls.