BD PharmingenTM Purified Recombinant Annexin V

Description

Flow cytometric analysis of Annexin V staining and blocking. Jurkat T cells were induced to undergo apoptosis by treatment with anti-Fas antibody, clone DX2 Cat. No. 555670 and Protein G for three hours first & second panels . Other cultures were left untreated third & fourth panels . Cells were incubated with FITC Annexin V first & third panels or with recombinant Annexin V to block Annexin V binding sites, and then with FITC Annexin V second & fourth panels . After a three hour treatment with Fas mAb, a population of cells was FITC Annexin V positive first panel, M2 gate . FITC Annexin V staining was blocked when cells were first incubated with purified recombinant Annexin V second panel . As expected, the cell population that was not treated with Fas mAb were primarily Annexin V negative third panel . The small number of Annexin V positive cells in the untreated population likely represents a basal level of apoptosis. This was blocked when cells were first incubated with purified recombinant Annexin V fourth panel . The M1 and M2 gates demarcate FITC Annexin V negative and positive populations, respectively. The addition of Protein G enhances the ability of DX2 to induce apoptosis, presumably by crosslinking the Fas receptor. Analysis of purified recombinant Annexin V 1 mug by SDS/PAGE. Gel was stained with Commasie Blue. Flow cytometric analysis of Annexin V staining and blocking. Jurkat T cells were induced to undergo apoptosis by treatment with anti-Fas antibody, clone DX2 Cat. No. 555670 and Protein G for three hours first & second panels . Other cultures were left untreated third & fourth panels . Cells were incubated with FITC Annexin V first & third panels or with recombinant Annexin V to block Annexin V binding sites, and then with FITC Annexin V second & fourth panels . After a three hour treatment with Fas mAb, a population of cells was FITC Annexin V positive first panel, M2 gate . FITC Annexin V staining was blocked when cells were first incubated with purified recombinant Annexin V second panel . As expected, the cell population that was not treated with Fas mAb were primarily Annexin V negative third panel . The small number of Annexin V positive cells in the untreated population likely represents a basal level of apoptosis. This was blocked when cells were first incubated with purified recombinant Annexin V fourth panel . The M1 and M2 gates demarcate FITC Annexin V negative and positive populations, respectively. The addition of Protein G enhances the ability of DX2 to induce apoptosis, presumably by crosslinking the Fas receptor. Show More Analysis of purified recombinant Annexin V 1 mug by SDS/PAGE. Gel was stained with Commasie Blue. Show More Flow cytometric analysis of Annexin V staining and blocking. Jurkat T cells were induced to undergo apoptosis by treatment with anti-Fas antibody, clone DX2 Cat. No. 555670 and Protein G for three hours first & second panels . Other cultures were left untreated third & fourth panels . Cells were incubated with FITC Annexin V first & third panels or with recombinant Annexin V to block Annexin V binding sites, and then with FITC Annexin V second & fourth panels . After a three hour treatment with Fas mAb, a population of cells was FITC Annexin V positive first panel, M2 gate . FITC Annexin V staining was blocked when cells were first incubated with purified recombinant Annexin V second panel . As expected, the cell population that was not treated with Fas mAb were primarily Annexin V negative third panel . The small number of Annexin V positive cells in the untreated population likely represents a basal level of apoptosis. This was blocked when cells were first incubated with purified recombinant Annexin V fourth panel . The M1 and M2 gates demarcate FITC Annexin V negative and positive populations, respectively. The addition of Protein G enhances the ability of DX2 to induce apoptosis, presumably by crosslinking the Fas receptor. Analysis of purified recombinant Annexin V 1 mug by SDS/PAGE. Gel was stained with Commasie Blue.