BD PharmingenTM Recombinant Human p53 Protein (Wildtype)

Description

Analysis of purified recombinant p53. Lane 1, Coomassie Blue stained 4-20 % mini SDS/PAGE. Lanes 2 and 3, Western blot analysis of p53 using clone PAb 240 Cat. No. 554166 or a mouse IgG1 isotype negative control. A major p53 band is identified at 53 kDa. A lower molecular weight doublet, reactive with anti-p53 antibody, is uncharacterized. 1 mug of purified p53 was loaded into each lane of the gel. MW, Coomassie Blue stained molecular weight standards. Western blot analysis of recombinant p53 using a panel of antibodies. 150 ng of recombinant protein per lane of a mini gel was separated by SDS/PAGE and detected by Western blotting using p53 monoclonal antibodies that recognize different epitopes. A mouse IgG1 isotype was used as a negative control Clone A85-1, Cat. No. 553440 . The DO-1 and DO-7 antibodies identified a single band at 53 kDa. The other p53 antibodies identified a band at 53 kDa, as well as a lower uncharacterized band which may be a p53 breakdown product. Lane 1: G59-12, lane 2: PAb 122, lane 3: PAb240, lane 4: DO-1, lane 5: DO-7, lane 6: PAb 1801. Western blot analysis of a p53 titration. Decreasing amounts of recombinant human p53 were loaded onto lanes of a mini gel separated by SDS/PAGE and detected by Western blotting using a monoclonal antibody to human p53 clone DO-7, Cat. No. 554294 . Protein loaded per lane ranged from 600-19 ng 1, 600 ng , 2 300 ng , 3 150 ng , 4 75 ng , 5 38 ng , and 6 19 ng . Analysis of purified recombinant p53. Lane 1, Coomassie Blue stained 4-20 % mini SDS/PAGE. Lanes 2 and 3, Western blot analysis of p53 using clone PAb 240 Cat. No. 554166 or a mouse IgG1 isotype negative control. A major p53 band is identified at 53 kDa. A lower molecular weight doublet, reactive with anti-p53 antibody, is uncharacterized. 1 mug of purified p53 was loaded into each lane of the gel. MW, Coomassie Blue stained molecular weight standards. Show More Western blot analysis of recombinant p53 using a panel of antibodies. 150 ng of recombinant protein per lane of a mini gel was separated by SDS/PAGE and detected by Western blotting using p53 monoclonal antibodies that recognize different epitopes. A mouse IgG1 isotype was used as a negative control Clone A85-1, Cat. No. 553440 . The DO-1 and DO-7 antibodies identified a single band at 53 kDa. The other p53 antibodies identified a band at 53 kDa, as well as a lower uncharacterized band which may be a p53 breakdown product. Lane 1: G59-12, lane 2: PAb 122, lane 3: PAb240, lane 4: DO-1, lane 5: DO-7, lane 6: PAb 1801. Show More Western blot analysis of a p53 titration. Decreasing amounts of recombinant human p53 were loaded onto lanes of a mini gel separated by SDS/PAGE and detected by Western blotting using a monoclonal antibody to human p53 clone DO-7, Cat. No. 554294 . Protein loaded per lane ranged from 600-19 ng 1, 600 ng , 2 300 ng , 3 150 ng , 4 75 ng , 5 38 ng , and 6 19 ng . Show More Analysis of purified recombinant p53. Lane 1, Coomassie Blue stained 4-20 % mini SDS/PAGE. Lanes 2 and 3, Western blot analysis of p53 using clone PAb 240 Cat. No. 554166 or a mouse IgG1 isotype negative control. A major p53 band is identified at 53 kDa. A lower molecular weight doublet, reactive with anti-p53 antibody, is uncharacterized. 1 mug of purified p53 was loaded into each lane of the gel. MW, Coomassie Blue stained molecular weight standards. Western blot analysis of recombinant p53 using a panel of antibodies. 150 ng of recombinant protein per lane of a mini gel was separated by SDS/PAGE and detected by Western blotting using p53 monoclonal antibodies that recognize different epitopes. A mouse IgG1 isotype was used as a negative control Clone A85-1, Cat. No. 553440 . The DO-1 and DO-7 antibodies identified a single band at 53 kDa. The other p53 antibodies identified a band at 53 kDa, as well as a lower uncharacterized band which may be a p53 breakdown product. Lane 1: G59-12, lane 2: PAb 122, lane 3: PAb240, lane 4: DO-1, lane 5: DO-7, lane 6: PAb 1801. Western blot analysis of a p53 titration. Decreasing amounts of recombinant human p53 were loaded onto lanes of a mini gel separated by SDS/PAGE and detected by Western blotting using a monoclonal antibody to human p53 clone DO-7, Cat. No. 554294 . Protein loaded per lane ranged from 600-19 ng 1, 600 ng , 2 300 ng , 3 150 ng , 4 75 ng , 5 38 ng , and 6 19 ng .