BD PharmingenTM Transcription Factor Buffer Set

Description

Multicolor flow cytometric analysis of transcription factors expressed in different cell types using the BD Pharmingen Transcription Factor Buffer Set. Bivariate flow cytometric plots showing A CD4 versus FoxP3 expression in human peripheral blood lymphocytes PBL ; B IL-17A versus RORgt expression in BALB/c mouse Th17-polarized cells; C CD95 versus Bcl-6 expression in C57BL/6 mouse lymph node cells LNC and identification of germinal center B-cells using CD4-B220+IgDloCD95hi phenotype as green colorized dots; D Pax-6 versus Sox-2 in H9 WiCell, Madison, Wi human embryonic stem cell ESC derived neural cultures; E mouse CD25 versus Foxp3 expression in CD4 T cells derived from C57BL/6 mouse LNC; F CD4 versus T-bet expression in human PBL. Plots were derived from gated events with the forward and side light-scattering characteristics of intact lymphocytes or indicated cell types using BD FACSDiva Software v. 6.1.3 and a BD LSRFortessa Flow Cytometer System. Overview of Buffer Dilution and Staining Protocol. Multicolor flow cytometric analysis of transcription factors expressed in different cell types using the BD Pharmingen Transcription Factor Buffer Set. Bivariate flow cytometric plots showing A CD4 versus FoxP3 expression in human peripheral blood lymphocytes PBL ; B IL-17A versus RORgt expression in BALB/c mouse Th17-polarized cells; C CD95 versus Bcl-6 expression in C57BL/6 mouse lymph node cells LNC and identification of germinal center B-cells using CD4-B220+IgDloCD95hi phenotype as green colorized dots; D Pax-6 versus Sox-2 in H9 WiCell, Madison, Wi human embryonic stem cell ESC derived neural cultures; E mouse CD25 versus Foxp3 expression in CD4 T cells derived from C57BL/6 mouse LNC; F CD4 versus T-bet expression in human PBL. Plots were derived from gated events with the forward and side light-scattering characteristics of intact lymphocytes or indicated cell types using BD FACSDiva Software v. 6.1.3 and a BD LSRFortessa Flow Cytometer System. Show More Overview of Buffer Dilution and Staining Protocol. Show More Multicolor flow cytometric analysis of transcription factors expressed in different cell types using the BD Pharmingen Transcription Factor Buffer Set. Bivariate flow cytometric plots showing A CD4 versus FoxP3 expression in human peripheral blood lymphocytes PBL ; B IL-17A versus RORgt expression in BALB/c mouse Th17-polarized cells; C CD95 versus Bcl-6 expression in C57BL/6 mouse lymph node cells LNC and identification of germinal center B-cells using CD4-B220+IgDloCD95hi phenotype as green colorized dots; D Pax-6 versus Sox-2 in H9 WiCell, Madison, Wi human embryonic stem cell ESC derived neural cultures; E mouse CD25 versus Foxp3 expression in CD4 T cells derived from C57BL/6 mouse LNC; F CD4 versus T-bet expression in human PBL. Plots were derived from gated events with the forward and side light-scattering characteristics of intact lymphocytes or indicated cell types using BD FACSDiva Software v. 6.1.3 and a BD LSRFortessa Flow Cytometer System. Overview of Buffer Dilution and Staining Protocol.